Abstract
Probing the baculovirus infection process is essential in optimizing recombinant protein production. Typically, researchers monitor the infection process in stirred reactors that contain cells that have been infected at different times after virus inoculation, particularly if cells pass the primary infection and become infected by progeny virus. This chapter describes several alternative bioreactor systems for baculovirus infection. We provide an example alternative system that holds promise to avoid asynchronous distributions in infection time. Namely, we describe a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production.
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Wu, HC., Hu, YC., Bentley, W.E. (2016). Tubular Bioreactor for Probing Baculovirus Infection and Protein Production. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_23
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DOI: https://doi.org/10.1007/978-1-4939-3043-2_23
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3042-5
Online ISBN: 978-1-4939-3043-2
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