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Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a piggyBac Transposon Delivery System and Temporal Control of Transgene Expression

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Cell Reprogramming

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1330))

Abstract

The discovery of induced pluripotent stem cells (iPSCs) has had a transforming effect on our understanding of biology and has brought an enormous promise to regenerative medicine. It has opened up a magnitude of unprecedented possibilities to study disease processes in vitro, model them in animal systems, and develop patient-specific cell-based regenerative therapies. iPSCs derived from other than the human species will be instrumental for bringing these prospects to fruition by providing preclinical models and novel treatments for veterinary medicine. In this chapter, we describe the derivation of iPSCs from equine embryonic fibroblasts using a non-viral method developed in our laboratory and originally applied to the murine and human systems (Woltjen et al., Nature 458:766–770, 2009). We will detail the procedures involved and discuss potential pitfalls as well as elaborate on possible variations and future improvements of this technique.

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Author information

Authors and Affiliations

  1. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 60 Murray St., Toronto, ON, Canada, M5T 3X7

    Kristina Nagy & Andras Nagy

  2. Department of Obstetrics and Gynecology, University of Toronto, Toronto, ON, Canada

    Andras Nagy

  3. Lunenfeld-Tanenbaum, Research Institute, Mount Sinai Hospital, Joseph & Wolf Lebovic Health Complex, 600 University Ave., Toronto, ON, Canada, M5G 1X5

    Andras Nagy

Authors
  1. Kristina Nagy
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  2. Andras Nagy
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Corresponding author

Correspondence to Andras Nagy .

Editor information

Editors and Affiliations

  1. Stem Cell and Genetic Engineering Group, Department of Materials Engineering Faculty of Engineering, Monash University, Clayton, Victoria, Australia

    Paul J. Verma

  2. Swinburne University of Technology, Hawthorn, Victoria, Australia

    Huseyin Sumer

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Nagy, K., Nagy, A. (2015). Derivation of Equine-Induced Pluripotent Stem Cell Lines Using a piggyBac Transposon Delivery System and Temporal Control of Transgene Expression. In: Verma, P., Sumer, H. (eds) Cell Reprogramming. Methods in Molecular Biology, vol 1330. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2848-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-2848-4_8

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2847-7

  • Online ISBN: 978-1-4939-2848-4

  • eBook Packages: Springer Protocols

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