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Cell Reprogramming

Methods and Protocols

  • Paul J. Verma
  • Huseyin Sumer

Part of the Methods in Molecular Biology book series (MIMB, volume 1330)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Background

  3. De Novo Reprogramming

  4. Livestock, Domestic and Endangered Species

    1. Front Matter
      Pages 55-55
    2. Luis F. Malaver-Ortega, Amir Taheri-Ghahfarokhi, Huseyin Sumer
      Pages 57-68
    3. Yangqing Lu, Franklin D. West, Brian J. Jordan, Robert B. Beckstead, Erin T. Jordan, Steven L. Stice
      Pages 89-99
    4. Inbar Friedrich Ben-Nun, Susanne C. Montague, Marlys L. Houck, Oliver Ryder, Jeanne F. Loring
      Pages 101-109
  5. Germ-Line Transmission of Pluripotent Stem Cells

    1. Front Matter
      Pages 111-111
    2. Martha C. Gómez, C. Earle Pope
      Pages 133-152
    3. Franklin D. West, Steve L. Terlouw, John R. Dobrinsky, Yangqing Lu, Erin T. Jordan, Steven L. Stice
      Pages 153-167
    4. S. M. Hosseini, F. Moulavi, M. H. Nasr-Esfahani
      Pages 169-188
  6. Influencing Reprogramming and Genome Editing

    1. Front Matter
      Pages 203-203
    2. William Lee, Richard D. W. Kelly, Ka Yu Yeung, Gael Cagnone, Matthew McKenzie, Justin C. St. John
      Pages 219-252
    3. Amir Taheri-Ghahfarokhi, Luis F. Malaver-Ortega, Huseyin Sumer
      Pages 253-267
  7. Back Matter
    Pages 269-271

About this book

Introduction

This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells, and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates;  alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell Reprogramming: Methods and Protocols is the perfect guide for molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

Keywords

Cellular Reprogramming Technology Disease Modeling Germ Line Transmission Mitocondrial DNA Nuclear Reprogramming Pluripotent Stem Cells Somatic Cells

Editors and affiliations

  • Paul J. Verma
    • 1
  • Huseyin Sumer
    • 2
  1. 1.Stem Cell and Genetic Engineering Group, Department of Materials Engineering Faculty of EngineeringMonash UniversityClaytonAustralia
  2. 2.Swinburne University of TechnologyHawthornAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2848-4
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2847-7
  • Online ISBN 978-1-4939-2848-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site