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Electron Tomography Methods for C. elegans

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C. elegans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1327))

Abstract

Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fiducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.

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Acknowledgements

We thank Ken Nguyen and Willisa Liou for help in tissue preparation and operation of the Technai20 microscope, and Ashleigh Bouchelion and Emily Semaya for annotation of the tomograms shown in Figs. 2, 3, and 4. Chris Crocker created Fig. 1. Kevin Fisher provided technical help with Amira. Access to NYSBC facilities has been supported by the Albert Einstein College of Medicine. We also thank NIH OD 010943 for support to DHH. Data collected at NYSBC was made possible by a grant from NYSTAR. The NYSBC facility was constructed with support from Research Facilities Improvement Program Grant C06 RR017528 from the NIH National Center for Research Resources.

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Authors and Affiliations

  1. Albert Einstein College of Medicine, Center for C. elegans Anatomy, 1410 Pelham Parkway South, Room 601, Bronx, NY, 10461, USA

    David H. Hall

  2. New York Structural Biology Center, New York, NY, USA

    William J. Rice

Authors
  1. David H. Hall
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  2. William J. Rice
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Corresponding author

Correspondence to David H. Hall .

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Editors and Affiliations

  1. Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA

    David Biron

  2. Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, New Jersey, USA

    Gal Haspel

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Hall, D.H., Rice, W.J. (2015). Electron Tomography Methods for C. elegans . In: Biron, D., Haspel, G. (eds) C. elegans. Methods in Molecular Biology, vol 1327. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4939-2842-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2842-2_11

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4939-2841-5

  • Online ISBN: 978-1-4939-2842-2

  • eBook Packages: Springer Protocols

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