Abstract
Reconstituted thymus organ culture is based on fetal thymus organ culture (FTOC). Purified thymocyte populations, from genetically modified mice or even from other species, are cultured in vitro with thymic lobes depleted of their endogenous thymocytes (by 2′-deoxyguanosine treatment) to form a new thymus. This potent and timesaving method is distinct from FTOC, which assesses development of unmodified thymic lobes, and reaggregate thymic organ culture, in which epithelial cells are separately purified before being aggregated with thymocytes.
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Acknowledgements
We thank Graham Anderson (University of Birmingham) for advice on RTOC.
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Deng, Z., Liu, H., Rui, J., Liu, X. (2016). Reconstituted Thymus Organ Culture. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_13
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DOI: https://doi.org/10.1007/978-1-4939-2809-5_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2808-8
Online ISBN: 978-1-4939-2809-5
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