Abstract
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.
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Acknowledgements
This work was supported by the Swedish Cancer Society. SJ is a Kempe Foundation scholarship recipient.
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Jia, S., Marjavaara, L., Buckland, R., Sharma, S., Chabes, A. (2015). Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection. In: Vengrova, S., Dalgaard, J. (eds) DNA Replication. Methods in Molecular Biology, vol 1300. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2596-4_8
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DOI: https://doi.org/10.1007/978-1-4939-2596-4_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2595-7
Online ISBN: 978-1-4939-2596-4
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