Abstract
The RNome of a cell is highly diverse and consists besides messenger RNAs (mRNAs), transfer RNAs (tRNAs), and ribosomal RNAs (rRNAs) also of other small and long transcript entities without apparent coding potential. This class of molecules, commonly referred to as non-protein-coding RNAs (ncRNAs), is involved in regulating numerous biological processes and thought to contribute to cellular complexity. Therefore, much effort is put into their identification and further functional characterization. Here we provide a cost-effective and reliable method for cDNA library construction of small RNAs in the size range of 20–500 residues. The effectiveness of the described method is demonstrated by the analysis of ribosome-associated small RNAs in the eukaryotic model organism Trypanosoma brucei.
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Acknowledgement
This research was partly supported by the NCCR “RNA & Disease” funded by the Swiss National Science Foundation. Additional grant support derives from the Swiss National Science Foundation (31003A_143388/1) and the Austrian Science Fund FWF (project number: Y315) to N.P.
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Gebetsberger, J., Fricker, R., Polacek, N. (2015). cDNA Library Generation for the Analysis of Small RNAs by High-Throughput Sequencing. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 1296. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2547-6_13
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DOI: https://doi.org/10.1007/978-1-4939-2547-6_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2546-9
Online ISBN: 978-1-4939-2547-6
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