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Sugarcane (Saccharum Spp. Hybrids)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

Abstract

Genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. We describe here Agrobacterium tumefaciens-mediated transformation of sugarcane. Embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent Agrobacterium strain AGL1 carrying a constitutive nptII expression cassette in vector pPZP200. Selection with 30 mg/L geneticin during the callus phase and 30 mg/L paromomycin during regeneration of shoots and roots effectively suppressed the development of non-transgenic plants. This protocol was successful with a commercially important sugarcane cultivar, CP-88-1762, at a transformation efficiency of two independent transgenic plants per g of callus.

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Authors and Affiliations

  1. Agronomy Department, Plant Molecular and Cellular Biology Program, Genetics Institute, University of Florida, 3085 McCarty Hall, 110500, Gainesville, FL, 32611-0500, USA

    Hao Wu & Fredy Altpeter

Authors
  1. Hao Wu
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  2. Fredy Altpeter
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Corresponding author

Correspondence to Fredy Altpeter .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Wu, H., Altpeter, F. (2015). Sugarcane (Saccharum Spp. Hybrids). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_24

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_24

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

  • eBook Packages: Springer Protocols

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