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New Analytical Strategies Applied to the Determination of Coenzyme Q10 in Biological Matrix

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Advanced Protocols in Oxidative Stress III

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1208))

Abstract

In the last few years the importance of Coenzyme Q10 (CoQ10) determination has gained clinical relevance. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier for the production of cellular energy. In addition, it is recognized as a primary regenerating antioxidant playing an intrinsic role against oxidative damage. There are some reports of low CoQ10 levels in a number of disorders, such as cancer, muscular, neurodegenerative, cardiological, and reproductive diseases. Therefore, it is a priority to develop analytical methodologies for evaluating CoQ10 in matrices of greater importance for the correct diagnosis of diseases, simple enough to be used in routine clinical laboratories.

In this chapter two recently developed techniques, capillary electrophoresis and microHPLC, for the analysis of CoQ10 in biological matrices, are studied.

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Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires, Buenos Aires, Argentina

    Mario Contin, Sabrina Flor & Manuela Martinefski

  2. Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina

    Silvia Lucangioli & Valeria Tripodi

  3. Department of Pharmaceutical Technology, Faculty of Pharmacy and Biochemistry, Universidad de Buenos Aires, 956 Junin Street, 5th Floor, CABA, 1113, Capital Federal, Buenos Aires, Argentina

    Valeria Tripodi

Authors
  1. Mario Contin
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  2. Sabrina Flor
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  3. Manuela Martinefski
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  4. Silvia Lucangioli
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  5. Valeria Tripodi
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Corresponding author

Correspondence to Valeria Tripodi .

Editor information

Editors and Affiliations

  1. University of Florida, Gainsville, Florida, USA

    Donald Armstrong

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Contin, M., Flor, S., Martinefski, M., Lucangioli, S., Tripodi, V. (2015). New Analytical Strategies Applied to the Determination of Coenzyme Q10 in Biological Matrix. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress III. Methods in Molecular Biology, vol 1208. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1441-8_29

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  • DOI: https://doi.org/10.1007/978-1-4939-1441-8_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1440-1

  • Online ISBN: 978-1-4939-1441-8

  • eBook Packages: Springer Protocols

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