Abstract
Lectin-probed western blot analysis, the so-called lectin blot analysis, is a useful method to yield basic information on the glycan structures of glycoproteins, based on the carbohydrate-binding specificities of lectins. By lectin blot analysis, researchers can directly analyze the glycan structures without releasing the glycans from glycoproteins. Here, the author describes protocols for standard analysis, and applies analysis in combination with glycosidase digestion of blot.
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Acknowledgement
The author thanks Ms. Riho Tange in his laboratory for her excellent technical assistance.
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Sato, T. (2014). Lectin-Probed Western Blot Analysis. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_8
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DOI: https://doi.org/10.1007/978-1-4939-1292-6_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1292-6
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