Methods and Protocols

  • Jun Hirabayashi

Part of the Methods in Molecular Biology book series (MIMB, volume 1200)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Standard Techniques

    1. Front Matter
      Pages 1-1
    2. Els J. M. Van Damme
      Pages 3-13
    3. Yuka Kobayashi, Hirokazu Kawagishi
      Pages 15-38
    4. Haruko Ogawa, Kimie Date
      Pages 39-45
    5. Kotone Sano, Haruko Ogawa
      Pages 47-52
    6. Takeshi Sato
      Pages 93-100
    7. Yuka Kobayashi
      Pages 121-129
    8. Kenta Moriwaki, Eiji Miyoshi
      Pages 147-152
    9. Yoshihiro Akimoto, Hayato Kawakami
      Pages 153-163
    10. Yoichi Takeda, Ichiro Matsuo
      Pages 207-214
  3. Emerging Techniques for Lectin-Based Glycomics

  4. Techniques for Elucidating Functions of Endogenous Animal Lectins

  5. Structural Biology and Engineering of Lectins

  6. Comprehensive Lists

    1. Front Matter
      Pages 553-553

About this book


Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.


conventional hemagglutiniation core protein glycans glycoprotein histochemical analyses isothermal calorimetry lectin-probed western blot quantitative interaction analyses serial lectin-affinity chromatography procedure surface plasmon resonance

Editors and affiliations

  • Jun Hirabayashi
    • 1
  1. 1.National Institute of Advanced Industrial Science and TechnologyTsukubaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1291-9
  • Online ISBN 978-1-4939-1292-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site