Abstract
Analysis of animal models of disease is essential to the understanding of human disease and the identification of potential targets for clinical drugs. Global analysis of proteins by mass spectrometry is an important tool for these studies. Stable isotope labeling in mammals (SILAM) was developed to quantitate the proteomes of rodents using mass spectrometry. The crux of SILAM analysis is the complete labeling of all proteins in a rodent with heavy nitrogen (15N). These 15N tissues are then employed as an internal standard for quantitative proteomics analysis using a high-resolution and mass-accuracy mass spectrometer.
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Acknowledgements
We acknowledge funding from NIH grants R01 MH067880 and P41 RR011823. We also like to thank Dr. Claire Delahunty for her comments on the manuscript.
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McClatchy, D.B., Yates, J.R. (2014). Stable Isotope Labeling in Mammals (SILAM). In: Martins-de-Souza, D. (eds) Shotgun Proteomics. Methods in Molecular Biology, vol 1156. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0685-7_8
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DOI: https://doi.org/10.1007/978-1-4939-0685-7_8
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