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Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL)

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Shotgun Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1156))

Abstract

Proteomics has been revolutionized by the rapid advance of mass spectrometric instrumentations and techniques. Parallel methodologies for the quantification of proteomes also evolved, including in vitro stable isotope labeling. Here, we present a protocol for employing isotope-coded protein labeling (ICPL) as part of a shotgun proteomics workflow denoting its advantages and disadvantages. This protocol is suitable to studying any proteome of interest, only requiring a specific sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders.

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Author information

Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil

    Daniel Martins-de-Souza

  2. Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Kraepelinstr. 2, Munich, 80804, Germany

    Giuseppina Maccarrone, Maria Lebar & Daniel Martins-de-Souza

  3. Research Group of Proteomics, Department of Psychiatry and Psychotherapy, Ludwig-Maximilians-University of Munich (LMU), Nußbaumstrasse 7, Munich, 80336, Germany

    Daniel Martins-de-Souza

  4. Laboratory of Neurosciences (LIM-27), Institute of Psychiatry, University of Sao Paulo, Sao Paulo, Brazil

    Daniel Martins-de-Souza

Authors
  1. Giuseppina Maccarrone
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  2. Maria Lebar
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  3. Daniel Martins-de-Souza
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Corresponding author

Correspondence to Daniel Martins-de-Souza .

Editor information

Editors and Affiliations

  1. Ludwig-Maximilians-University, Munich, Germany

    Daniel Martins-de-Souza

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Maccarrone, G., Lebar, M., Martins-de-Souza, D. (2014). Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL). In: Martins-de-Souza, D. (eds) Shotgun Proteomics. Methods in Molecular Biology, vol 1156. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0685-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-0685-7_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0684-0

  • Online ISBN: 978-1-4939-0685-7

  • eBook Packages: Springer Protocols

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