Abstract
Extracts taken from spent growth media from Pseudomonas aeruginosa can be analyzed for N-acyl-l-homoserine lactones and 2-alkyl-4-(1H)-quinolones (AQs), including the known quorum sensing signalling molecules of P. aeruginosa, in a specific and sensitive manner by liquid chromatography coupled with tandem mass spectrometric detection. This analysis can be conducted in a quantitative manner by comparison with matrix-matched calibration samples.
Catharine A. Ortori and Nigel Halliday are joint first authors.
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Acknowledgements
The contribution of Mavis Daykin to the development of this methodology is gratefully acknowledged as is the work of Ram Chhabra and Alex Truman in synthesizing AHLs and AQs. The work was supported by grants from the Biotechnology and Biological Sciences Research Council (BBSRC grant number BBS/B/09740, BB/C/509082, and P18575) and the Medical Research Council (MRC grant number G0801558).
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Ortori, C.A., Halliday, N., Cámara, M., Williams, P., Barrett, D.A. (2014). LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_21
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_21
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