Skip to main content
SpringerLink
Log in

Participation of Hepatic Parenchymal and Kupffer Cells in Chylomicron and Cholesterol Metabolism

  • Chapter
The Reticuloendothelial System and Atherosclerosis

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1))

Abstract

The administration of reticuloendothelial (RE) stimulants produced an increased growth and functional activity of RE cells. Parenchymal cell number, structure, and function, as indicated by BSP removal, and histological and electron-microscopic observations were unaltered in RE-stimulated rats. The RE hyperactive group manifested an increased intravascular removal of colloidal carbon which was associated with enhanced accumulation of carbon in Kupffer cells.

The technique of selectively and exclusively producing an RE hyper-functional state was employed to evaluate the metabolic behavior of labeled chyle, chylomicrons, and an artificial lipid emulsion, designated as “RE test lipid emulsion.” The intravascular removal of chyle and chylomicrons was not enhanced in RE-stimulated rats. The predominant uptake of injected chyle and chylomicrons was hepatic in nature with lung and spleen manifesting 1-3% removal. Cholesterol-containing chylomicrons demonstrated a behavior similar to that of triglyceride-containing chylomicrons and unlike colloidal carbon and the RE test lipid emulsion. Significant differences existed, however, between the organ uptake of cholesterol and triglyceride-labeled chylomicrons.

An artificial lipid emulsion was developed that possessed an intravascular behavior and an organ distribution which was comparable to colloidal and particulate materials which are removed by phagocytosis. This RE test lipid emulsion has been employed in the evaluation of RE function in experimental and clinical subjects.

Normal chylomicrons containing cholesterol or triglyceride are relatively immune to RE cell uptake and the hepatic mechanism of chylomicron removal involves essentially their removal by the parenchymal cell.

These studies were supported in part by the USPHS (HE-05367) and the Atomic Energy Commission.

These studies were conducted during the tenure of a USPHS Predoctoral Fellowship.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. S. H. Gage, Cornell Vet., 10: 154, 1920.

    Google Scholar 

  2. G. Gulliver, Gerber’s General Anatomy, 1847, p. 88.

    Google Scholar 

  3. S. H. Gage and P.A. Fish, Am. J. Anat., 34: 1, 1924.

    Article  CAS  Google Scholar 

  4. V.P. Dole and J.T. Hamlin, III, Physiol. Rev., 42: 674, 1962.

    PubMed  CAS  Google Scholar 

  5. T. Olivecrona, E.P. George, and B. Borgstrom, Federation Proc., 20: 928, 1961.

    Google Scholar 

  6. R.E. Olson and J. W. Vester, Physiol. Rev., 40: 677, 1960.

    PubMed  CAS  Google Scholar 

  7. B. Borgstrom and N. Tryding, Acta Physiol. Scand., 37: 127, 1956.

    Article  PubMed  CAS  Google Scholar 

  8. R.J. Havel, Am. J. Clin. Nutr., 6: 662, 1958.

    PubMed  CAS  Google Scholar 

  9. C.B. Laurell, Scand. J. Clin. Lab. Invest., 6: 22, 1954.

    Article  PubMed  CAS  Google Scholar 

  10. B. Borgstrom, in: K. Bloch, Ed., Lipide Metabolism. New York, John Wiley and Sons, 1960, p. 128.

    Google Scholar 

  11. J. E. French and B. Morris, J. Physiol., 138: 326, 1957.

    PubMed  CAS  Google Scholar 

  12. R.J. Havel and D. S. Fredrickson, J.Clin.Invest., 35: 1025, 1956.

    Article  PubMed  CAS  Google Scholar 

  13. J.E. French and B. Morris, J. Physiol., 140: 262, 1958.

    PubMed  CAS  Google Scholar 

  14. B. Borgstrom, P. Wlodawer, and C. Naito, Digestion, Absorption Intestinale et Transport des Glycerides Chez Les Animaux Superieurs. Paris, Centre National de la Recherche Scientifique, 1961, p. 125.

    Google Scholar 

  15. T. Olivecrona, J. Lipid Res., 3: 439, 1962.

    CAS  Google Scholar 

  16. T. Olivecrona, Acta Physiol. Scand., 55: 170, 1962.

    Article  PubMed  CAS  Google Scholar 

  17. J. H. Bragdon and R.S. Gordon, J. Clin. Invest., 37: 574, 1958.

    Article  PubMed  CAS  Google Scholar 

  18. R. J. Havel and A. Golfien, J. Lipid Res., 2: 389, 1961.

    CAS  Google Scholar 

  19. P. Belfrage, B. Borgstrom, and T. Olivecrona, Acta Physiol. Scand., 58: 111, 1963.

    Article  PubMed  CAS  Google Scholar 

  20. D.S. Goodman, J. Clin.Invest., 41: 1886, 1962.

    Article  PubMed  CAS  Google Scholar 

  21. R. Daoust: R.W. Brauer, Ed., Liver Function. Washington, D. C., Am. Inst. Biol. Sciences, 1958, p. 3.

    Google Scholar 

  22. R.H. Jaffe and S. L. Berman, Arch. Pathol. Lab. Med., 5: 1020, 1928.

    Google Scholar 

  23. R. H. Jaffe, in: H. Downey, Ed., Handbook of Hematology. New York, P.B. Hoeber, 1938, p. 977.

    Google Scholar 

  24. M. Friedman, S.O. Byers, and S. St. George, Am.J.Physiol., 184: 141, 1956.

    PubMed  CAS  Google Scholar 

  25. S.O. Byers, S. St. George, and M. Friedman, in: B.N. Halpern, Ed., Physiopathology of the Reticuloendothelial System. Springfield, Illinois, Charles C. Thomas, 1957, p. 128.

    Google Scholar 

  26. T. Neveu, G. Biozzi, B. Benacerraf, C. Stiffel, and B.N. Halpern, Am.J.Physiol., 187: 269, 1956.

    PubMed  CAS  Google Scholar 

  27. S.J. Thannhauser, Lipidoses. New York, Oxford University Press, 1950.

    Google Scholar 

  28. N.R. DiLuzio, Ann.N.Y.Acad.Sci., 88: 244, 1960.

    CAS  Google Scholar 

  29. N.R. DiLuzio, Nature, 185: 616, 1960.

    Article  CAS  Google Scholar 

  30. N.R. DiLuzio, J. Houston, and E.E. Elko, in: S. Garattini and R. Paoletti, Eds., Drugs Affecting Lipid Metabolism. Amsterdam, Elsevier Publishing Co., 1961, p. 228.

    Google Scholar 

  31. S.J. Riggi and N.R. DiLuzio, J. Lipid Res., 3: 339, 1962.

    CAS  Google Scholar 

  32. N. R. DiLuzio and S.J. Riggi, J. Reticuloendothelial Soc., 3: 135, 1966.

    CAS  Google Scholar 

  33. W.R. Waddell, B.P. Geyer, E. Clarke, and F.J. Stare, Am.J. Physiol., 177: 90, 1954.

    PubMed  CAS  Google Scholar 

  34. R.G. Murray and S. Freeman, J. Lab. Clin. Med., 38: 56, 1951.

    PubMed  CAS  Google Scholar 

  35. J. Van Den Bosch, E. Evrard, A. Billiau, J.V. Joossens, and P. DeSomer, J. Exptl. Med., 114: 1035, 1961.

    Article  Google Scholar 

  36. C. T. Ashworth, V.A. Stembridge, and E. Sanders, Am. J. Physiol., 198: 1326, 1960.

    PubMed  CAS  Google Scholar 

  37. C. T. Ashworth, N. R. DiLuzio, and S.J. Riggi, Exptl. Mol. Pathol. Suppl., 1: 83, 1963.

    Google Scholar 

  38. G. Biozzi, B. Bencerraf, and B. N. Halpern, Brit. J. Exptl. Pathol., 34: 441, 1953.

    CAS  Google Scholar 

  39. N.R. DiLuzio, J.Am.Oil Chemists’ Soc., 37: 163, 1960.

    Article  CAS  Google Scholar 

  40. N. R. DiLuzio, K.A. Simon, and A. C. Upton, Arch. Pathol., 64: 649, 1957.

    CAS  Google Scholar 

  41. D.A. Blickens and N.R. DiLuzio, J. Reticuloendothelial Soc., 1: 68, 1964.

    Google Scholar 

  42. B. Benacerraf and M.M. Sebestyen, Federation Proc., 16: 860, 1957.

    Google Scholar 

  43. L.S. Kelly, E.L. Dobson, C.R. Finney, and J.D. Hirsch, Am.J. Physiol., 198: 1134, 1960.

    PubMed  CAS  Google Scholar 

  44. S.J. Riggi and N.R. DiLuzio, Am.J.Physiol., 200: 297, 1961.

    PubMed  CAS  Google Scholar 

  45. S.J. Riggi and N.R. DiLuzio, Nature, 193: 1292, 1962.

    Article  PubMed  CAS  Google Scholar 

  46. W.R. Wooles and N.R. DiLuzio, J. Reticuloendothelial Soc., 1: 160, 1941.

    Google Scholar 

  47. J.L. Bollman, J.C. Cain, and J.H. Grindlay, J. Lab. Clin. Med., 33: 1349, 1948.

    PubMed  CAS  Google Scholar 

  48. N.R. DiLuzio and S.J. Riggi, J. Reticuloendothelial Soc., 1: 248, 1964.

    Google Scholar 

  49. N.K. Salky, N.R. DiLuzio, D.P. P’Pool, and A.J. Sutherland, J. Am. Med. Ass., 187: 744, 1964.

    Article  CAS  Google Scholar 

  50. N.R. DiLuzio, N.K. Salky, S.J. Riggi, and A.J. Ladman, in: The Reticuloendothelial System, Morphology, Immunology and Regulation. Kyoto, Japan, “Nissba” Printing Co., 1965, p. 389.

    Google Scholar 

  51. N.R. DiLuzio and S.J. Riggi, J. Reticuloendothelial Soc., 1: 136, 1964.

    Google Scholar 

  52. V. Aberdeen, P.A. Shepherd, and W.J. Simmonds, Quart. J. Exptl. Physiol., 45: 265, 1960.

    CAS  Google Scholar 

  53. I. Toro, P. Ruzsa, and P. Rohlich, Exptl. Cell Res., 26: 601, 1962.

    Article  PubMed  CAS  Google Scholar 

  54. M. Kojima and Y. Imai, Proc. Japan Soc. Reticuloendothelial System, 1: 61, 1961.

    Google Scholar 

  55. W.J. Lossow, N. Brot, and I. L. Chaikoff, J. Lipid Res., 3: 207, 1962.

    CAS  Google Scholar 

  56. C. Markowitz and F.C. Mann, Am.J.Physiol., 93: 521, 1930.

    CAS  Google Scholar 

  57. Y. Stein and B. Shapiro, J. Lipid Res., 1: 326, 1960.

    PubMed  CAS  Google Scholar 

  58. D. L. Berliner and T. F. Dougherty, in: J. H. Heller, Ed., Reticuloendothelial Structure and Function. New York, Ronald Press Co., 1960, p. 403.

    Google Scholar 

  59. T. F. Dougherty and D. L. Berliner, in: G. Pincus, Ed., Conference on Hormones and Atherosclerosis. New York, Academic Press, Inc., 1959, p. 103.

    Google Scholar 

  60. R. Jones, W.A. Thomas, and R. F. Scott, J. Exptl. and Mol. Pathol., 1: 65, 1962.

    Article  CAS  Google Scholar 

  61. P.J. Nestel and R.O. Scow, J. Lipid Res., 5: 46, 1964.

    CAS  Google Scholar 

  62. E. L. Bierman, E. Gordis, and J. T. Hamlin, III, J. Clin. Invest., 41 2254, 1962.

    Article  PubMed  CAS  Google Scholar 

  63. N. R. DiLuzio and E. L. Bierman, Proc. Soc. Exptl. Biol. and Med., 116: 1045, 1964.

    CAS  Google Scholar 

  64. B. Morris and J. E. French, Quart.J. Exptl. Physiol., 43: 180, 1958.

    CAS  Google Scholar 

  65. B. Edgren and B. Ivemark, Acta Physiol.Scand., 48: 402, 1960.

    Article  PubMed  CAS  Google Scholar 

  66. P. Belfrage, B. Edgren, and T. Olivecrona, Acta Physiol. Scand., 62: 344, 1964.

    Article  PubMed  CAS  Google Scholar 

  67. B. Morris and F.C. Courtice, Quart. J. Exptl. Physiol., 41: 341, 1956.

    CAS  Google Scholar 

  68. R. Reiser, M.C. Williams, and M. F. Sorrels, J. Lipid Res., 1: 241, 1960.

    PubMed  CAS  Google Scholar 

  69. B. Borgstrom and P. Jordan, Acta Soc. Med. Upsalien., 64: 185, 1959.

    CAS  Google Scholar 

  70. D. S. Fredrickson, D. L. McCollester, and K. Ono, J. Clin.Invest., 37: 1333, 1958.

    Article  PubMed  CAS  Google Scholar 

  71. B. Shapiro, Digestion, Absorption Intestinale et Transport des Glycerides Chez Les Animaux Superieurs. Paris, Centre National de la Recherche Scientifique, 1961, p. 165.

    Google Scholar 

  72. D.W. Fawcett, J. Nat. Cancer Inst., 15: 1475, 1955.

    PubMed  CAS  Google Scholar 

  73. J. C. Hampton, Texas Rep.Biol. and Med., 18: 602, 1960.

    Google Scholar 

  74. G. Holle, German Med. Monthly, 7: 91, 1962.

    Google Scholar 

  75. J.M. Felts and P.A. Mayes, Nature, 206: 195, 1965.

    Article  PubMed  CAS  Google Scholar 

  76. J.M. Felts, Ann. N. Y. Acad. Sci., 131: 24, 1965.

    Article  PubMed  CAS  Google Scholar 

  77. J.A. Higgins and C. Green, Biochem.J., 99: 631, 1966.

    PubMed  CAS  Google Scholar 

  78. N.K. Salky, D. Mills, and N.E. DiLuzio, J. Lab. and Clin. Med., 66: 952, 1965.

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. S. J. Riggi

    Present address: Lederle Laboratories, Pearl River, New York, USA

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Tennessee Medical Units, Memphis, Tennessee, USA

    N. R. Di Luzio & S. J. Riggi

Authors
  1. N. R. Di Luzio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. J. Riggi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Physiology and Biophysics, University of Tennessee Medical Units, Memphis, Tennessee, USA

    Nicholas R. Di Luzio (President) (President)

  2. Institute of Pharmacology, University of Milan, Milan, Italy

    Rodolfo Paoletti

Rights and permissions

Reprints and permissions

Copyright information

© 1967 Springer Science+Business Media New York

About this chapter

Cite this chapter

Di Luzio, N.R., Riggi, S.J. (1967). Participation of Hepatic Parenchymal and Kupffer Cells in Chylomicron and Cholesterol Metabolism. In: Luzio, N.R.D., Paoletti, R. (eds) The Reticuloendothelial System and Atherosclerosis. Advances in Experimental Medicine and Biology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7796-2_34

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-7796-2_34

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-7798-6

  • Online ISBN: 978-1-4684-7796-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation