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Electrophysiology of Clonal Nerve Cell Lines

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Excitable Cells in Tissue Culture

Abstract

In recent years it has become apparent that excitable cells grown in tissue culture provide some of the most advantageous preparations for investigating fundamental questions in neurobiology. Among the different in vitro neuronal systems presently available (for reviews, see Sato, 1973; Nelson, 1975; Fischbach and Nelson, 1977; Patrick et al., 1978; Patterson, 1978; Federoff and Hertz, 1977), clonal cell lines permit important types of experimentation that are impossible in other preparations in vivo or in vitro. The unique features of cell lines include: (1) the ability to multiply indefinitely with fairly consistent phenotypes from generation to generation; (2) the ability to differentiate in response to changes in culture conditions; (3) the feasibility of genetic manipulations such as somatic cell hybridization; (4) the ease in obtaining large quantities of homogeneous material for biochemical experiments; (5) the unusually large size of individual cells in some neuronal lines which makes them particularly accessible to detailed analysis of electrically and chemically activated ionic conductances; and (6) the possibility of obtaining large cells by artificial fusion.

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Authors and Affiliations

  1. Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, 20205, USA

    Ilan Spector

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  1. Ilan Spector
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Editors and Affiliations

  1. Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205, USA

    Phillip G. Nelson

  2. Department of Physiology, Duke University Medical Center, Durham, North Carolina, 27710, USA

    Melvyn Lieberman

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© 1981 Plenum Press, New York

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Spector, I. (1981). Electrophysiology of Clonal Nerve Cell Lines. In: Nelson, P.G., Lieberman, M. (eds) Excitable Cells in Tissue Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3803-1_7

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  • DOI: https://doi.org/10.1007/978-1-4684-3803-1_7

  • Publisher Name: Springer, Boston, MA

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