Abstract
Since the pioneering work of Astwood (1), prolactin has been considered the major luteotrophic agent in the rat. Maintenance of rat luteal function in vitro beyond 6–8 days has been difficult (2,3). The relationship between luteal function and the role played by the other pituitary gonadotrophins, steroids and placental hormones is not entirely understood. This is further complicated by the apparent fluctuations in the levels of the luteotrophic hormones required for in vivo maintenance of luteal function (6, 7, 10, 11). The present study was undertaken to determine the hormone requirements for luteinization and long-term maintenance of rat granulosa cell cultures. An attempt was made to reconcile the apparent conflicting results from in vivo and in vitro maintenance of luteal function.
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© 1979 Plenum Press, New York
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Centola, G.M. (1979). Hormone Requirements for Long-Term Maintenance of Rat Granulosa Cell Cultures. In: Channing, C.P., Marsh, J.M., Sadler, W.A. (eds) Ovarian Follicular and Corpus Luteum Function. Advances in Experimental Medicine and Biology, vol 112. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3474-3_27
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DOI: https://doi.org/10.1007/978-1-4684-3474-3_27
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