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Attachment Factors in Cell Culture

  • Chapter
Mammalian Cell Culture

Abstract

Cells in vivo are subject to several classes of environmental factors that affect proliferative and differentiative potential. Among these are nutrients, hormones, binding proteins that modulate the actions of nutrients and hormones, extracellular enzymes, and extracellular matrix, stroma or basement membranes (Barnes and Sato, 1980b,c). Although the importance of the extracellular substratum in vivo in the regulation of cellular growth and differentiation has been recognized for some time (Hay, 1981), only recently has it become possible to explore in vitro the complicated and often subtle relationships between cells and substrata and to examine the effects of cell-substratum interactions. Several important insights led to improved experimental design of investigations into the role of the cell substratum in culture. First, it was recognized that components of the serum supplement to conventional culture media provided a substratum for cells in culture, and that the nature and effects of these and other factors affecting cell differentiation and proliferation could best be examined in serum-free cell culture (Fisher et al., 1958; Grinnell, 1978; Barnes and Sato, 1980b,c). Secondly, cells in culture were discovered to synthesize and deposit extracellular matrix components that sometimes are organized in elaborate and complex simulations in vitro of basement membrane or interstitial stroma in vivo (Hay, 1981; Kleinman et al., 1981).

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References

  • Adamsonh, E. D., Gaunt, S. J., and Graham, C. F., 1979, The differentiation of teratocarcinoma stem cells is marked by the types of collagen which are synthesized. Cell 17: 469–476.

    Article  Google Scholar 

  • Alitalo, K., Kurkinen, M., and Vaheri, A., 1980a, Extracellular matrix components synthesized by human amniotic epithelial cells in culture. Cell 19: 1053–1062.

    Article  PubMed  CAS  Google Scholar 

  • Alitalo, K., Kurkinen, M., Vaheri, A., Virtanen, H., Rohde, H., and Timpl, R., 1980b, Basal lamina glycoproteins are produced by neuroblastoma cells. Nature (London) 287: 465–466.

    Article  CAS  Google Scholar 

  • Alitalo, K., Keski-Oja, J., and Vaheri, A., 1981, Extracellular matrix proteins characterize human tumor cell lines. Int J Cancer 27: 755–761.

    Article  PubMed  CAS  Google Scholar 

  • Alitalo, K., Kuismanen, E., Myllyla, R., Kustala, U., Askoseljavaara, S., and Vaheri, A., 1982, Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells. J Cell Biol 94: 497–505.

    Article  PubMed  CAS  Google Scholar 

  • Barnes, D. W., 1982a, Epidermal growth factor inhibits growth of A431 human epidermoid carcinoma in serum-free cell culture. J Cell Biol 93: 1–4.

    Article  PubMed  CAS  Google Scholar 

  • Barnes, D. W., 1982b, Growth of A–431 human epidermoid carcinoma in serum-free cell culture: Inhibition by epidermal growth factor, Cold Spring Harbor Conferences on Cell Proliferation 9: 937–941.

    Google Scholar 

  • Barnes, D. W., and Sato, G., 1979, Growth of a human mammary tumor cell line in a serum-free medium. Nature (London) 281: 388–389.

    Article  CAS  Google Scholar 

  • Barnes, D. W., and Sato, G., 1980a, Factors that stimulate proliferation of breast cancer cells in vitro in serum-free medium, in: Cell Biology of Breast Cancer ( C. M. McGrath, M. J. Brennan, and M. A. Rich), Academic Press, New York, pp. 277–287.

    Google Scholar 

  • Barnes, D. W., and Sato, G., 1980b, Methods for growth of cultured cells in serum-free medium. Anal Biochem. 102: 255–270.

    Article  PubMed  CAS  Google Scholar 

  • Barnes, D. W., and Sato, G., 1980c, Serum-free cell culture: A unifying approach. Cell 22: 649–655.

    Google Scholar 

  • Barnes, D. W., and Silnutzer, J., 1983, Isolation of human serum spreading factor. J Biol Chem. 258: 12548–12552.

    PubMed  CAS  Google Scholar 

  • Barnes, D. W., Wolfe, R., Serrero, G., McClure, D., and Sato, G., 1980, Effects of a serum spreading factor on growth and morphology of cells in serum-free medium. J Supramol Struct Cell Biochem. 14: 47–63.

    CAS  Google Scholar 

  • Barnes, D. W., van der Bosch, J., Masui, H., Miyazaki, K., and Sato, G., 1981, The culture of human tumor cells in serum-free medium, in: Methods in Enzymology, Volume 79, Interferons, Part B ( S. Pestka, ed.), Academic Press, New York, pp. 368–391.

    Google Scholar 

  • Barnes, D. W., Darmon, M., and Orly, J., 1982, Serum-spreading factor: Effects on RF1 rat ovary cells and 1003 mouse embryonal carcinoma cells in serum-free media. Cold Spring Harbor Conferences on Cell Proliferation 9: 155–167.

    CAS  Google Scholar 

  • Barnes, D. W., Silnutzer, J., See, C., and Shaffer, M., 1983, Characterization of human serum spreading factor with monoclonal antibody. Proc Natl Acad Sci USA. 80: 1362–1366.

    Article  PubMed  CAS  Google Scholar 

  • Barnes, D. W., Mousetis, L., Amos, B. and Silnutzer, J., 1984b, Glass bead affinity chromatography of cell attachment and spreading promoting factors of human serum. Analytical Biochemistry 137: 196–204.

    Article  PubMed  CAS  Google Scholar 

  • Basara, M. L., McCarthy. J B., Barnes, D. W., and Furcht, L. T., 1984, Tumor cell migration to serum-spreading factor, Fed. Proc. (in press).

    Google Scholar 

  • Benecke, B., Ben-Ze’ev, A., and Penman, S., 1980, The regulation of RNA metabolism in suspended and reattached anchorage-dependent 3T6 fibroblasts. J Cell Physiol. 103: 247–254.

    Article  PubMed  CAS  Google Scholar 

  • Bensusan, H. B., Koh, T. L., Henry, K. G., Murray, B. A., and Culp, L. A., 1978, Evidence that fibronectin is the collagen receptor on platelet membranes. Proc Natl Acad Sci USA. 75: 5864–5868.

    Article  PubMed  CAS  Google Scholar 

  • Ben-Ze’ev, A., Farmer, S. R., and Penman, S., 1980, Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts. Cell 21: 365–372.

    Article  PubMed  Google Scholar 

  • Birdwell, C. R., Gospodarowicz, D., and Nicolson, G. L., 1978, Identification, localization, and role of fibronectin in cultures of bovine endothelial cells. Proc Natl Acad Sci USA. 75: 3273–3277.

    Article  PubMed  CAS  Google Scholar 

  • Burrill, P. H., Bernardini, I., Kleinman, H. K., and Kretchmer, N., 1981, Effect of serum, fibronectin, and laminin on adhesion of rabbit intestinal epithelial cells in culture. J Supramol Struct Cell Biochem. 16: 385–392.

    Article  PubMed  CAS  Google Scholar 

  • Carlsson, R., Engvall, E., Freeman, A., and Ruoslahti, E., 1981, Laminin and fibronectin in cell adhesion: Enhanced adhesion of cells from regenerating liver to laminin. Proc Natl Acad Sci USA. 78: 2403–2406.

    Google Scholar 

  • Chandrasekhar, S., and Millis, A.J. T., 1979, Fibronectin from aged fibroblasts is defective in promoting cellular adhesion. J Cell Physiol. 103: 47–54.

    Article  Google Scholar 

  • Chen, L. B., Gallimore, P. H., and McDougall. J K., 1976, Correlation between tumor induction and the large external transformation sensitive protein on the cell surface. Proc Natl Acad Sci USA. 73: 3570–3574.

    Article  PubMed  CAS  Google Scholar 

  • Chen, L. B., Burridge, K., Murray, A., Walsh, M. L., Copple, C. D., Bushnell, A., McDougall. J K., and Gallimore, P. H., 1978a, Modulation of cell surface glycocalyx: Studies on large, external transformation-sensitive protein. Ann NY Acad Sci. 312: 366–381.

    Google Scholar 

  • Chen, L. B., Murray, A., Segal, R. A., Bushnell, A., and Walsh, M. L., 1978b, Studies on intercellular LETS glycoprotein matrices. Cell 14: 377–391.

    Article  PubMed  CAS  Google Scholar 

  • Chung, A. E., Jaffe, R., Freeman, I. L., Vergnes, J., Braginski. J E., and Carlin, B., 1979, Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line. Cell 16: 277–287.

    CAS  Google Scholar 

  • Civerchia-Perez, L., Faris, B., LaPointe, G., Beldekas, J., Leibowitz, H., and Franablau, C., 1980, Use of collagen-hydroxyethylmethacrylate hydrogels for cell growth. Proc Natl Acad Sci USA. 77: 2064–2068.

    Article  PubMed  CAS  Google Scholar 

  • Cooper, A. R., Kurkinen, M., Taylor, A., and Hogan, B. L. M., 1981, Studies on the biosynthesis of laminin by murine parietal endoderm cells. Eur J Biochem. 119: 189–197.

    Article  PubMed  CAS  Google Scholar 

  • Couchman. J R., Hook, M., Rees, D. A., and Timpl, R., 1983, Adhesion, growth, and matrix production by fibroblasts on laminin substrates.. J Cell Biol 96: 177–183.

    Article  Google Scholar 

  • Courtoy, P. J., Kanmar, Y. S., Nynes, R. O., and Farquhar, M. G., 1980, Fibronectin localization in the rat glomerulus. J Cell Biol 87: 691–696.

    Article  PubMed  CAS  Google Scholar 

  • Crouch, E., Bornstein, P., and Canfield, T., 1978a, Collagen synthesis by human amniotic fluid cells in culture: Characterization of a procollagen with three identical proal(I) chains. Biochemistry 17: 5499–5509.

    Google Scholar 

  • Crouch, E., Balian, G., Holbrook, K., Duksin, D., and Bornstein, P., 1978b, Amniotic fluid fibronectin: Characterization and synthesis by cells in culture. J Cell Biol 78: 701–715.

    Google Scholar 

  • Gulp, L. A., Barrett. J R., Buniel, J., and Hitri, S., 1978, Two functionally distinct pools of glycosaminoglycan in the substrate adhesion site of murine cells. J Cell Biol 79: 788–800.

    Google Scholar 

  • Darmon, M. Y., 1982, Laminin provides a better substrate than fibronectin for attachment, growth, and differentiation of 1003 embryonal carcinoma cells. In vitro 18: 997–1003.

    Article  PubMed  CAS  Google Scholar 

  • Del Rosso, M., Cappelletti, R., Viti, M., Vannucchi, S., and Chiarugi, V., 1981, Binding of the basement-membrane glycoprotein laminin to glycosaminoglycans. Biochem J. 199: 699–704.

    PubMed  Google Scholar 

  • Dessau, W., Timpl, R., Jilek, F., and von der Mark, K., 1978, Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. J Cell Biol 79: 342–355.

    Article  PubMed  CAS  Google Scholar 

  • Dessau, W., Vertel, B. M., von der Mark, H., and von der Mark, K., 1981, Extracellular matrix formation by chondrocytes in monolayer culture. J Cell Biol 90: 78–83.

    Article  PubMed  CAS  Google Scholar 

  • Ekblom, P., Alitalo, K., Vaheri, A., Timpl, R., and Saxen, L., 1980, Induction of a basement membrane glycoprotein in embryonic kidney: Possible role of laminin in morphogenesis. Proc Natl Acad Sci USA. 77: 485–489.

    Google Scholar 

  • Emerman. J T., and Pitelka, D. R., 1977, Maintenance and induction of morphological differentiation in dissociated mammary epithelium on floating collagen membranes. In vitro 13: 316–328.

    Article  Google Scholar 

  • Engel, J., Odermatt, E., Engel, A., Madri. J A., Furthmayr, H., Rohde, H., and Timpl, R., 1981, Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150: 97–120.

    CAS  Google Scholar 

  • Engvall, E., and Ruoslahti, E., 1977, Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer 20: 1–5.

    Article  PubMed  CAS  Google Scholar 

  • Engvall, E., Ruoslahti, E., and Miller, E. J., 1978, Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med. 147: 1584–1595.

    Article  PubMed  CAS  Google Scholar 

  • Fisher, H. W., Puck, T. T., and Sato, G., 1958, Molecular growth requirements of single mammalian cells: The action of fetuin in promoting cell attachment to glass. Proc Natl Acad Sci USA. 44: 4–10.

    Google Scholar 

  • Florini. J R., and Roberts, S. B., 1979, A serum-free medium for the growth of muscle cells in culture. In vitro 15: 983–992.

    Article  Google Scholar 

  • Folkman, J., 1977, Conformational control of cell and tumor growth. Recent Adv Cancer Res. 1: 119–130.

    Google Scholar 

  • Folkman, J., and Moscona, A., 1978, Role of cell shape in growth control. Nature (London) 273: 345–349.

    Article  CAS  Google Scholar 

  • Fujii, D. K., Massoglia, S. L., Savion, N., and Gospodarowicz, D., 1982, Neurite outgrowth and protein synthesis by PC 12 cells as a function of substratum and nerve growth factor. J Neurosci. 2: 1157–1175.

    PubMed  CAS  Google Scholar 

  • Furcht, L. T., Mosher, D. F., and Wendelschafer-Crabb, G., 1978, Immunocytochemical localization of fibronectin (LETS Protein) on the surface of L6 myoglasts: Light and electron microscopic studies. Cell 13: 263–271.

    Google Scholar 

  • Furcht, L. T., Mosher, D. F., Wendelschafer-Crabb, G., and Foidart, J., 1979, Reversal by glucocorticoid hormones of the loss of a fibronectin and procollagen miatrix around transformed human cells. Cancer Res. 39: 2077–2083.

    PubMed  CAS  Google Scholar 

  • Gerfaux, J., Chang-Fournier, F., Bardos, P., Mun, J., and Chang, C., 1979, Lectin-like activity of components extracted from human glomerular basement membrane. Proc Natl Acad Sci USA. 76: 5129–5133.

    Article  PubMed  CAS  Google Scholar 

  • Giguere, L., Cheng, J., and Gospodarowicz, D., 1982, Factors involved in the control of proliferation of bovine corneal endothelial cells maintained in serum-free medium. J Cell Physiol 110: 72–80.

    Article  PubMed  CAS  Google Scholar 

  • Gold, L. I., and Pearlstein, E., 1980, Fibronectin-collagen binding and requirement during cellular adhesion. Biochem J 186: 551–559.

    PubMed  CAS  Google Scholar 

  • Gospodarowicz, D., 1984, Preparation of extracellular matrices produced by cultured bovine corneal endothelial cells and PF-HR–9 endodermal cells; their use in cell culture, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato.), A. R. Liss Inc., New York (in press).

    Google Scholar 

  • Gospodarowicz, D., Greenberg, G., Birdwell, C. R., 1978, Determination of cellular shape by the extracellular matrix and its correlation with the control of cellular growth. Cancer Res. 38: 4155–4171.

    PubMed  CAS  Google Scholar 

  • Gospodarowicz, D., Vlodavsky, I., Greenberg, G., and Johnson, L. K., 1979, Cellular shape is determined by the extracellular matrix and is responsible for the control of cellular growth and function. Cold Spring Harbor Conferences on Cell Proliferation 6:561 - 592.

    Google Scholar 

  • Gospodarowicz, D., Delgado, D., and Vlodansky, I., 1980, Permissive effect of the extracellular matrix on cell proliferation in vitro. Proc Natl Acad Sci USA. 77: 4094–4098.

    Article  PubMed  CAS  Google Scholar 

  • Gospodarowicz, D., Greenberg, G., Foidart. J M., and Savion, N., 1981, The production and localization of laminin in cultured vascular and corneal endothelial cells. J Cell Physiol 197: 171–183.

    Google Scholar 

  • Gospodarowicz, D., Lui, G. M., and Gonzalez, R., 1982, High-density lipoproteins and the proliferation of human tumor cells maintained on extracellular matrix-coated dishes and exposed to defined medium. Cancer Res. 42: 3704–3713.

    PubMed  CAS  Google Scholar 

  • Gospodarowicz, D., Gonzalez, R., and Fujii, D. K., 1983, Are factors originating from serum, plasma, or cultured cells involved in the growth-promoting effect of the extracellular matrix produced by cultured bovine corneal endothelial cells?. J Cell Physiol 114: 191–202.

    Article  PubMed  CAS  Google Scholar 

  • Green, J., Todaro, G. J., and Goldberg, B., 1966, Collagen synthesis in fibroblasts transformed by oncogenic viruses. Nature (London) 209: 916–917.

    Article  CAS  Google Scholar 

  • Grinnell, F., 1976. Cell spreading factor. Exp Cell Res. 102: 51–62.

    Article  PubMed  CAS  Google Scholar 

  • Grinnell, F., 1978, Cellular adhesiveness and extracellular substrata, in: International Review of Cytology, Volume 53 ( Bourne, G. H., Danielli. J F., and Jean, K. W.), Academic Press, New York, pp. 65–144.

    Google Scholar 

  • Grinnell, F., 1980, Fibroblast receptor for cell-substratum adhesion: Studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin). J Cell Biol 86: 104–112.

    Google Scholar 

  • Grinnell, F., and Feld, M. K., 1979, Initial adhesion of human fibroblasts in serum-free medium: Possible role of secreted fibronectin. Cell 17: 117–129.

    Google Scholar 

  • Grinnell, F., and Feld, M. K., 1980, Spreading of human fibroblasts in serum-free medium: Inhibition by dithiothreitol and the effect of cold insoluble globulin (plasma fibronectin). J Cell Physiol 104: 321–334.

    Google Scholar 

  • Grinnell, F., and Feld, M. K., 1982, Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium. J Biol Chem. 257: 4888–4893.

    PubMed  CAS  Google Scholar 

  • Grinnell, F., and Hays, D. G., 1978. Cell adhesion and spreading factor. Exp Cell Res. 115: 221–229.

    Article  PubMed  CAS  Google Scholar 

  • Grinnell, F., and Minter, D., 1978, Attachment and spreading of baby hamster kidney cells to collagen substrata: Effects of cold-insoluble globulin. Proc Natl Acad Sci USA. 75: 4408–4412.

    Google Scholar 

  • Grotendorst, G. R., Seppa, H. E. J., Kleinman, H. K., and Martin, G. R., 1981, Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci USA. 78: 3669–3672.

    Article  PubMed  CAS  Google Scholar 

  • Grotendorst, G. R., Kleinman, H. K., Rohrbach, D. H., Hewitt, A. T., Varner, H. H., Horigan, E. A., HasScil. J R., Terranova, V. P., and Martin, G. R., 1982, Role of attachment factors in mediating the attachment, distribution, and differentiation of cells, in: Growth of Cells in Hormonally Defined Media ( G. H. Sato, A. B. Pardee, and D. A. Sirbasku), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 403–413.

    Google Scholar 

  • Harper, P. A., and Juliano, R. L., 1980, Isolation and characterization of Chinese hamster ovary cell variants defective in adhesion to fibronectin-coated collagen. J Cell Biol 87: 755–763.

    Article  PubMed  CAS  Google Scholar 

  • Harper, P. A., and Juliano, R. L., 1981, Two distinct mechanisms of fibroblast adhesion. Nature (London) 290: 136–138.

    Article  CAS  Google Scholar 

  • Hauschka, S. D., and Königsberg, I. R., 1966, The influence of collagen on the development of muscle clones. Proc Natl Acad Sci USA. 55: 119–126.

    Article  PubMed  CAS  Google Scholar 

  • Hay, E. D., 1981, Extracellular matrix. J Cell Biol 91: 205s–223s.

    Article  PubMed  CAS  Google Scholar 

  • Hayashi, M., and Yamada, K. M., 1981, Differences in domain structures between cellular and plasma fibronectins. J Biol Chem. 256: 11292–11301.

    PubMed  CAS  Google Scholar 

  • Hayman, E. G., and Ruoslahti, E., 1979, Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix. J Cell Biol 83: 255–259.

    Article  PubMed  CAS  Google Scholar 

  • Hayman, E. G., Engvall, E., and Ruoslahti, E., 1981, Concomitant loss of cell surface fibronectin and laminin from transformed rat kidney cells. J Cell Biol 88: 352–357.

    Article  PubMed  CAS  Google Scholar 

  • Hayman, E. G., Oldberg, A., Martin, G. R., and Ruoslahti, E., 1982a, Codistribution of heparin sulfate proteoglycan laminin, and fibronectin in the extracellular matrix of normal rat kidney cells and their coordinate absence in transformed cells. J Cell Biol 94: 28–35.

    Article  PubMed  CAS  Google Scholar 

  • Hayman, E. G., Engvall, E., A’Hearn, E., Barnes, D., Pierschbacher, M., and Ruoslahti, E., 1982b. Cell attachment on replicas of SDS Polyacrylamide gels reveals two adhesive plasma proteins. J Cell Biol 95: 20–23.

    Article  PubMed  CAS  Google Scholar 

  • Hayman, E. G., Pierschbacher, M. D., Ohgren, Y., and Ruoslahti, E., 1983, Serum spreading factor (vitromectin) is present at the cell surface and in tissues. Proc Natl Acad Sci. 80: 4003–4007.

    Article  PubMed  CAS  Google Scholar 

  • Hedman, K., Kurkinen, M., Alitalo, K., Vaheri, A., Johansson, S., Höök, M., 1979, Isolation of the pericellular matrix of human fibroblast cultures. Cell Biol 81: 83–91.

    Article  CAS  Google Scholar 

  • Hewitt, A. T., Kleinman, H. K., Pennypacker. J P., and Martin, G. R., 1980, Identification of an adhesion factor for chondrocytes. Proc Natl Acad Sci USA. 77: 385–388.

    Article  PubMed  CAS  Google Scholar 

  • Hewitt, A. T., Varner, H. H., Silver, M. H., Dessau, W., Wilkes, C. M., and Martin, G. R., 1982, The isolation and partial characterization of chondronectin, an attachment factor for chondrocytes. J Biol Chem. 257: 2330–2334.

    PubMed  CAS  Google Scholar 

  • Hogan, B. L. M., 1980, High molecular weight extracellular proteins synthesized by endoderm cells derived from mouse teratocarcinoma cells and normal extraembryonic membranes. Dev Biol 76: 275–285.

    Article  PubMed  CAS  Google Scholar 

  • Hogan, B. L. M., and Tilly, R., 1981. Cell interactions and endoderm differentiation in cultured mouse embryos. J Embryol Exp Morphol. 62: 379–394.

    PubMed  CAS  Google Scholar 

  • Holmes, R., 1967, Preparations from human serum of an alpha-one protein which induces the immediate growth of unadapted cells in vitro. J Cell Biol 32: 297–308.

    Article  PubMed  CAS  Google Scholar 

  • Holmes, R., Mercer, G., and Mohamed, N., 1979, Studies of a-protein in human cell cultures. In vitro 15: 522–531.

    Article  PubMed  CAS  Google Scholar 

  • Howe, C. C., and Solter, D., 1980, Identification of noncollagenous basement membrane glycopolypeptides synthesized by mouse parietal entoderm and an entodermal cell line. Dev Biol 77: 480–487.

    Article  PubMed  CAS  Google Scholar 

  • Hynes, R. O., 1973, Alteration of cell-surface proteins by viral transformation and by proteolysis. Proc Natl Acad Sci USA. 70: 3170–3174.

    Article  PubMed  CAS  Google Scholar 

  • Hynes, R. O., 1976. Cell surface protein and malignant transformation. Biochim Biophys Acta 458: 73–107.

    PubMed  CAS  Google Scholar 

  • Iverius, P., 1972, The interaction between human plasma lipoproteins and connective tissue glycosaminoglycans. J Biol Chem. 247: 2607–2613.

    PubMed  CAS  Google Scholar 

  • Jaffe, E. A., and Mosher, D. F., 1978, Synthesis of fibronectin by cultured human endothelial cells. J Exp Med. 147: 1779–1791.

    Article  PubMed  CAS  Google Scholar 

  • Johansson, S., and Hook, M., 1980, Heparin enhances the rate of binding of fibronectin to collagen. Biochem J 185: 521–524.

    Google Scholar 

  • Johansson, S., Kjellen, L., Hòòk, M., and Timpl, R., 1981, Substrate adhesion of rat hepatocytes: A comparison of laminin and fibronectin as attachment proteins. J Cell Biol 90: 260–264.

    Google Scholar 

  • John, H. A., and Lawson, H., 1980, The effect of different collagen types used as substrata on myogenesis in tissue culture. Cell Biol Int Rep. 4: 841–850.

    Article  PubMed  CAS  Google Scholar 

  • Keski-Oja, J., Gahmberg, C. G., Alitalo, K., 1982, Pericellular matrix and cell surface glycoproteins of virus-transformed mouse epithelial cells. Cancer Res. 42: 1147–1153.

    PubMed  CAS  Google Scholar 

  • Ketley. J N., Orkin, R. W., Martin, G. R., 1976, Collagen in developing chick muscle in vivo and in vitro. Exp Cell Res. 99: 261–268.

    Article  Google Scholar 

  • Kidwell, W. R., Wicha, M. S., Salomon, D., and Liotta, L. A., 1980, Differential recognition of basement membrane collagen by normal and neoplastic mammary cells, in: Cell Biology of Breast Cancer ( C. McGrath, M. J. Brennan, and M. A. Rich), Academic Press, New York, pp. 17–32.

    Google Scholar 

  • Kidwell, W. R., Salomon, D. S., Liotta, L. A., Zweibel. J A., and Bano, M., 1982, Effects of growth factors on mammary epithelial cell proliferation and basement-membrane synthesis, in: Growth of Cells in Hormonally Defined Media ( G. H. Sato, A. B. Pardee, and D. A. Sirbasku), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 807–818.

    Google Scholar 

  • Kidwell, W. R., Bano, M., and Salomon, D., 1984, The growth of normal mammary epithelium on collagen in serum-free medium, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. R. Liss Inc., New York (in press).

    Google Scholar 

  • Klebe, R. J., 1974, Isolation of a collage-dependent cell attachment factor. Nature (London) 250: 248–251.

    Article  CAS  Google Scholar 

  • Klebe, R. J., Hall. J R., Naylor, S. L., Dickey, W. D., 1978, Bioautography of cell attachment proteins. Exp Cell Res. 115: 73–78.

    CAS  Google Scholar 

  • Klebe, R. J., Bentley, K. L., Sasser, P. J., and Schoen, R. C., 1980, Elution of fibronectin from collagen with chaotropic agents. Exp Cell Res. 130: 111–117.

    Article  PubMed  CAS  Google Scholar 

  • Kleinman, H. K., McGoodwin, E. B., Martin, G. R., Klebe, R. J., Fietzek, P. P., and Woolley, D. E., 1978, Localization of the binding site for cell attachment in the a 1 ( I) chain of collagen. J Biol Chem. 253: 5642–5646.

    Google Scholar 

  • Kleinman, H. K., McGoodwin, E. B., Rennard, S. I., and Martin, G. R., 1979a, Preparation of collagen substrates for cell attachment: Effect of collagen concentration and phosphate buffer. Anal Biochem. 94: 308–312.

    Google Scholar 

  • Kleinman, H. K., Martin, G. R., and Fishman, P. H., 1979b, Ganglioside inhibition of fibronectin-mediated cell adhesion to collagen. Proc Natl Acad Sci USA. 76: 3367–3371.

    Article  PubMed  CAS  Google Scholar 

  • Kleinman, H. K., Klebe, R. J., and Martin, G. R., 1981, Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol 88: 473–485.

    Article  PubMed  CAS  Google Scholar 

  • Kumegawa, M., Hiramatsu, M., Yajima, T., Hatakeyama, K., Hosoda, S., and Namba, M., 1982, Effect of epidermal growth factor on collagen formation in liver-derived epithelial clone cells. Endocrinology 110: 607–612.

    Article  PubMed  CAS  Google Scholar 

  • Labat-Robert, J., Birembaut, P., Adnet. J J., Mercantini, F., and Robert, L., 1980, Loss of flbronectin in human breast cancer. Cell Biol Int Rep. 4 (6): 609–616.

    CAS  Google Scholar 

  • Lark, M. W., and Culp, L. A. 1982, Selective solubilization of hyaluronic acid from fibroblast substratum adhesion sites. J Biol Chem. 257: 14073–14080.

    PubMed  CAS  Google Scholar 

  • Ledbetter, S. R., Kleinman, H. K., Hassell. J R., and Martin, G. R., 1984, Methods for the isolation of laminin, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku and G. Sato), A. R. Liss Inc., New York, (in press).

    Google Scholar 

  • Leivo, I., Vaheri, A., Timpl, R., Wartiovaara, J., 1980, Appearance and distribution of collagen and laminin in the early mouse embryo. Dev Biol 76: 100–114.

    Article  PubMed  CAS  Google Scholar 

  • Leivo, I., Alitalo, K., Risteli, L., Vaheri, A., Timpl, R., Wartiovaara, J., 1982, Basal lamina glycoproteins laminin and type IV collagen are assembled into flve-fibered matrix in cultures of a tetracarcinoma-derived endodermal cell line. Exp Cell Res. 137: 15–23.

    Article  PubMed  CAS  Google Scholar 

  • Lieberman, I., Lamy, F., and Ove, P., 1959, Nonidentity of fetuin and protein growth (flattening) factor. Science 129: 43–44.

    Article  PubMed  CAS  Google Scholar 

  • Liesli, P., Dahl, D., and Vaheri, A., 1983, Laminin is produced by early rat ostrocytes in primary culture. J Cell Biol 96: 920–924.

    Article  Google Scholar 

  • Liotta, L. A., Shigeto, A., Robey, P. G., and Martin, G. R., 1979, Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor. Proc Natl Acad Sci USA. 76: 2268–2272.

    Article  PubMed  CAS  Google Scholar 

  • Liotta, L. A., Tryggvason, K., Garbisa, S., Hart, I., Foltz, C. M., and Shafie, S., 1980, Metastatic potential correlates with enzymatic degradation of basement membrane collage. Nature (London) 284: 67–68.

    Article  CAS  Google Scholar 

  • Marceau, N., Gayette, R., Valet. J P., and Deschenes, J., 1980, The effect of dexamethasone on formation of a flbronectin extracellular matrix by rat hepatocytes in vitro. Exp Cell Res. 125: 497–502.

    Article  PubMed  CAS  Google Scholar 

  • McDonald. J A., and Kelley, D. G., 1980, Degradation of flbronectin by human leukocyte elastase. J Biol Chem. 255: 8848–8858.

    Google Scholar 

  • Michalopoulos, G., and Pitot, H. C., 1975, Primary culture of parenchymal liver cells on collagen membranes. Exp Cell Res. 94: 70–78.

    Article  PubMed  CAS  Google Scholar 

  • Mosesson, M. W., and Umfleet, R. A., 1970, The cold-insoluble globulin of human plasma: I. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components. J Biol Chem. 245: 5728–5736.

    Google Scholar 

  • Mosher, D. F., Saksela, O., Keski-Oja, J., and Vaheri, A., 1977, Distribution of a major surface-associated glycoprotein, flbronectin, in cultures of adherent cells. J Supramol Struct Cell Biochem. 6: 551–557.

    CAS  Google Scholar 

  • Murakami, H., and Masui, H., 1980, Hormonal control of human colon carcinoma cell growth in serum-free medium. Proc Natl Acad Sci USA. 77: 3464–3468.

    Article  PubMed  CAS  Google Scholar 

  • Murray, B. A., Ansbacher, R., and Culp, L. A., 1980, Adhesion sites of murine fibroblasts on cold-insoluble globulin-adsorbed substrata.. J Cell Physiol 104: 335–348.

    Article  PubMed  CAS  Google Scholar 

  • Murray. J C., Stingl, G., Kleinman, H. K., Martin, G. R., and Katz, S. I., 1979, Epidermal cells adhere preferentially to type IV (basement membrane) collagen. J Cell Biol 80: 197–207.

    Article  Google Scholar 

  • Orly, J., and Sato, G., 1979, Fibronectin mediates cytokinesis and growth of rat follicular cells in serum-free medium. Cell 17: 295–305.

    Article  PubMed  CAS  Google Scholar 

  • Orly, J., Sato, G., and Erickson, G. F., 1980, Serum suppresses the expression of hormonally induced functions in cultured granulosa cells. Cell 20: 817–827.

    Article  PubMed  CAS  Google Scholar 

  • Ott, U., Odermatt, E., Engel, J., Furthmay, H., and Timpl, R., 1982, Protease resistance and conformation of laminin. Eur J Biochem. 123: 63–72.

    Article  PubMed  CAS  Google Scholar 

  • Pennypacker. J P., Hassell. J R., Yamada, K. M., and Pratt, R. M., 1979, The influence of an adhesive cell surface protein on chondrogenic expression in vitro. Exp Cell Res. 121: 411–415.

    Article  Google Scholar 

  • Pierschbacher, M. D., Hayman, E. G., and Ruoslahti, E., 1981, Location of the cell attachment site in fibronectin using monoclonal antibodies and proteolytic fragments of the molecule. Cell 26: 259–267.

    Article  PubMed  CAS  Google Scholar 

  • Pierschbacher, M. D., Hayman, E. G., and Ruoslahti, E., 1983, A synthetic peptide with the cell attachment activity of fibronectin. Proc Natl Acad Sci USA. 80: 1224–1227.

    Article  PubMed  CAS  Google Scholar 

  • Podleski, T. R., Greenberg, I., Schlessinger, J., and Yamada, K. M., 1979, Fibronectin delays the fusion of L6 myoblasts. Exp Cell Res. 122: 317–326.

    Article  PubMed  CAS  Google Scholar 

  • Postlethwaite, A. E., Seyer. J M., and Kang, A. H., 1978, Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagen-derived peptides. Proc Natl Acad Sci USA. 75: 871–875.

    Google Scholar 

  • Rao, C. N., Margulies, I. M. K., Tralka, T. S., Terranova, V. P., Madri. J A., and Liotta, L. A., 1982, Isolation of a subunit of laminin and its role in molecular structure and tumor cell attachment. J Biol Chem. 257: 9740–9744.

    CAS  Google Scholar 

  • Rennard, S. I., Berg, R., Martin, G. R., Foidart. J M., Robey, P. G., 1980, Enzyme-linked immunoassay ( ELISA) for connective tissue components. Anal Biochem. 104: 205–214.

    Google Scholar 

  • Risteli, L., and Timpl, R., 1981, Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes. Biochem J. 193: 749–755.

    PubMed  CAS  Google Scholar 

  • Rizzino, A., 1983, Two multipotent embryonal carcinoma cell lines irreversibly differentiate in defined media. Dev Biol 95: 126–136.

    Article  PubMed  CAS  Google Scholar 

  • Rizzino, A., 1984, The growth and differentiation of embryonal carcinoma cells in defined and serum-free media, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. R. Liss Inc., New York (in press).

    Google Scholar 

  • Rizzino, A., and Crowley, C., 1980, Growth and differentiation of embryonal carcinoma cell line F9 in defined media. Proc Natl Acad Sci USA. 77: 457–461.

    Article  PubMed  CAS  Google Scholar 

  • Rizzino, A., and Sato, G., 1978, Growth of embryonal carcinoma cells in serum-free medium. Proc Natl Acad Sci USA. 75: 1844–1848.

    Article  PubMed  CAS  Google Scholar 

  • Rizzino, A., and Sherman, M. I., 1979, Blastocysts in serum-free medium. Exp Cell Res. 121: 221–233.

    Article  PubMed  CAS  Google Scholar 

  • Rizzino, A., Terranova, V., Rohrbach, D., Crowley, C., and Rizzino, H., 1980, The effects of laminin on the growth and differentiation of embryonal carcinoma cells in defined media. J Supramol Struct Cell Biochem. 13: 243–253.

    CAS  Google Scholar 

  • Rohde, H., Wick, G., and Timpl, R., 1979, Immunochemical characterization of the basement membrane glycoprotein laminin. Eur J Biochem. 102: 195–201.

    Article  PubMed  CAS  Google Scholar 

  • Rojkind, M., Gatmaitan, Z., MacKensen, S., Giambrone, M., Ponce, P., and Reid, L. M., 1980, Connective tissue biomatrix: Its isolation and utilization for long-term cultures of normal rat hepatocytes. J Cell Biol 87: 225–263.

    Google Scholar 

  • Rollins, B. J., and Culp, L. A., 1979, Preliminary characterization of the proteoglycans in the substrata adhesion sites of normal and virus-transformed murine cells. Biochemistry 18: 5621–5629.

    Article  PubMed  CAS  Google Scholar 

  • Rubin, K., Hook, M., Obrink, B., Timpl, R., 1981, Substrate adhesion of rat hepatocytes: Mechanism of attachment to collagen substrates. Cell 24: 463–470.

    Google Scholar 

  • Ruoslahti, E., and Engvall, E., 1980, Complexing of fibronectin glycosaminoglycans and collagen. Biochim Biophys Acta 631: 350–358.

    Article  PubMed  CAS  Google Scholar 

  • Ruoslahti, E., and Vaheri, A., 1974, Novel human serum protein from fibroblast plasma membranes. Nature (London) 248: 789–791.

    Article  CAS  Google Scholar 

  • Ruoslahti, E., Hayman, E. G., Engvall, E., Cothran, W. C., and Butler, W. T., 1981, Alignment of biologically active domains in the fibronectin molecule. J Biol Chem. 256: 7277–7281.

    PubMed  CAS  Google Scholar 

  • Ruoslahti, E., Hayman, E. G., Pierschbacher, M., and Engvall, E., 1982, Fibronectin: Purification, immunochemical properties, and biological activities. Methods Enzymol 82: 803–831.

    Google Scholar 

  • Russo, J., Soule, H. D., McGrath, C., and Rich, M. A., 1976, Re-expression of the original tumor pattern by a human breast carcinoma cell line (MCF–7) in sponge culture. J Natl Cancer Inst. 56: 279–282.

    PubMed  CAS  Google Scholar 

  • Sakashita, S., and Ruoslahti, E., 1980, Laminin-like glycoproteins in extracellular matrix of endodermal cells. Arch Biochem Biophys. 205: 283–290.

    Article  PubMed  CAS  Google Scholar 

  • Sakashita, S., Engvall, E., and Ruoslahti, E., 1980, Basement membrane glycoprotein laminin binds to heparin. FEBS Lett. 116: 243–246.

    Article  PubMed  CAS  Google Scholar 

  • Salomon, D. S., Liotta, L. A., and Kidwell, W. R., 1981, Differential response to growth factor by rat mammary epithelium plated on different collagen substrata in serum-free medium. Proc Natl Acad Sci USA. 78: 382–386.

    Article  PubMed  CAS  Google Scholar 

  • Salomon, D. S., Bano, M., Smith, K. B., and Kidwell, W. R., 1982, Isolation and characterization of a growth factor (embryonin) from bovine fetuin which resembles x2-macroglobulin. J Biol Chem. 257: 14093–14101.

    PubMed  CAS  Google Scholar 

  • Salomon, D. S., Liotta, L. A., Panneerselvam, M., Terranova, V. P., Sahai, A., And Feknel, P., 1984a, Analysis of basement membrane synthesis and turnover in mouse embryonal and human A431 epidermoid carcinoma cells in serum-free medium, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. R. Liss Inc., New York (in press).

    Google Scholar 

  • Salomon, D. S., Smith, K. B., Losonczy, I., Bano, M., Kidwell, W. R., Alessandri, G., and Gullino, P. M., 1984b, a2-Macroglobulin, a contaminant of commercially prepared Pedersen fetuin: Isolation, characterization and biological activity, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. R. Liss Inc., New York (in press).

    Google Scholar 

  • Sandmeyer, S., Smith, R., Kiehn, D., Bornstein, P., 1981, Correlation of collagen synthesis in procollagen messenger RNA levels with transformation in rat embryo fibroblasts. Cancer Res. 41: 830–838.

    PubMed  CAS  Google Scholar 

  • Savion, N., Lui, G., Laherty, R., and Gospodarowicz, D., 1981, Factors controlling proliferation and progesterone production by bovine granulosa cells in serum-free medium. Endocrinology 109: 409–420.

    Article  PubMed  CAS  Google Scholar 

  • Shaffer, M. C., Foley, T. P., and Barnes, D. W., 1984, Quantitation of spreading factor in human biological fluids. J Lab Clin. Med. (in press).

    Google Scholar 

  • Shibata, S., Peters, B., Roberts, D., Goldstein, I., and Liotta, L., 1982, Isolation of laminin by affinity chromatography on immoblized Griffonia Simplicifolia I Lectin. FEBS Lett. 142: 194–198.

    Article  CAS  Google Scholar 

  • Sieber-Blum, M., Sieber, F., and Yamada, K. M., 1981, Cellular fibronectin promotes adrenergic differentiation of quail neural crest cells in vitro. Exp Cell Res. 133:285- 395.

    Google Scholar 

  • Silnutzer, J., and Barnes, D. W., 1984a, A biologically active thrombin cleavage product of human serum spreading factor. Biochem Biophys Res Comm. 118: 339–343.

    Article  PubMed  CAS  Google Scholar 

  • Silnutzer, J., and Barnes, D. W., 1984b, Human serum spreading factor: preparation, use and assay in cell culture, in: Cell Culture Methods for Molecular and Cell Biology (C. D. Barnes, D. Sirbasku and G. Sato),A. R. Liss, Inc., New York (in press).

    Google Scholar 

  • Sirica, A. E., Richards, W., Tsukada, Y., Sattler, C. A., and Pitot, H. C., 1979, Fetal phenotypic expression by adult rat hepatocytes on collagen gel/nylon meshes. Proc Natl Acad Sci USA. 76: 283–287.

    Article  PubMed  CAS  Google Scholar 

  • Smith. J C., Singh. J P., Lillquist. J S., Goon, D. S., and Stiles, C. D., 1982, Growth factors adherent to cell substrate are mitogenically active in situ. Nature (London) 296: 154–156.

    Article  Google Scholar 

  • Stathakis, N. E., and Mosesson, M. W., 1977, Interactions among heparin, cold-insoluble globulin, and fibrinogen in formation of the heparin-precipitable fraction of plasma. J Clin Invest. 60: 855–865.

    Article  PubMed  CAS  Google Scholar 

  • Stenman, S., Wartiovaara, J., and Vaheri, A., 1977, Changes in the distribution of a major fibroblast protein, fibronectin, during mitosis and interphase. J Cell Biol 74: 453–467.

    Article  PubMed  CAS  Google Scholar 

  • Stenman, T. S., Vaheri, A., Timpl, R., 1979, Changes in the matrix proteins, fibronectin and collagen, during differentiation of mouse tooth germ. Dev Biol 70: 116–126.

    Article  PubMed  Google Scholar 

  • Stenn, K. S., 1981, Epibolin: A protein of human plasma, that supports epithelial cell movement. Proc Natl Acad Sci USA. 78: 6907–6911.

    Google Scholar 

  • Stenn, K. S., 1984, Purification of epibolin from human plasma, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. R. Liss Inc., New York (in press).

    Google Scholar 

  • Stenn, K. S., Madri. J A., Roll, F. J., 1979, Migrating epidermis produces AB2 collagen and requires continual collagen synthesis for movement. Nature (London) 277: 229–232.

    CAS  Google Scholar 

  • Stenn, K. S., Madri. J A., Tinghitella, T., and Terranova, V. P., 1982, Multiple mechanisms of dissociated epidermal cell spreading. J Cell Biol 96: 63–67.

    Article  Google Scholar 

  • Strickland, S., Smith, K. K., Marotti, K. R., 1980, Hormonal induction of differentiation in teratocarcinoma stem cells: Generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347–365.

    Google Scholar 

  • Strom, S. C., and Michalopoulos, G., 1982, Collagen as a substrate for cell growth and differentiation, Methods Enzymol. 82: 544–555.

    Article  PubMed  CAS  Google Scholar 

  • Sun-Yang, N., Kube, D., Park, C., Furmanski, P., 1981, Growth of human mammary epithelial cells on collagen gel surfaces. Cancer Res 41: 4093–4100.

    Google Scholar 

  • Terranova, V. P., Rohibach, D. H., and Martin, G. R., 1980, Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen. Cell 22: 719–726.

    Article  PubMed  CAS  Google Scholar 

  • Timpl, R., Martin, G. R., Bruckner, P., Wick, G., and Wiedemann, H., 1978. Nature of the collagenous protein in a tumor basement membrane. Eur J Biochem. 84: 43–52.

    Article  PubMed  CAS  Google Scholar 

  • Timpl, R., Heilwig, R., Robey, P. G., Rennard, S. I., Foidart, J., and Martin, G. R., 1979, Laminin: A glycoprotein from basement membranes. J Biol Chem. 254: 9933–9937.

    Google Scholar 

  • Timpl, R., Rohde, H., Risteli, L., Ott, U., Roby, P. G., and Martin, G. R., 1982, Laminin. Methods Enzymol. 82: 831–838.

    CAS  Google Scholar 

  • Tseng, S. C. G., Savion, N., Gospodarowicz, D., and Stern, R., 1981, Characterization of collagens synthesized by cultured bovine corneal endothelial cells. J Biol Chem. 256: 3361–3365.

    PubMed  CAS  Google Scholar 

  • Tseng, S. C. G., Savion, N., Stern, R., and Gospodarowicz, D., 1982, Fibroblast growth factor modulates synthesis of collagen in cultured vascular endothelial cells. Eur J Biochem. 122: 355–360.

    Article  PubMed  CAS  Google Scholar 

  • Vaheri, A., Kurkinen, M., Lehto, V.-P., Linder, E., Timpl, R., 1978, Codistribution of pericellular matrix proteins in cultured fibroblasts and loss of transformation: Fibronectin and collagen. Proc Natl Acad Sci USA. 75: 4944–4948.

    Google Scholar 

  • Van der Bosch, J., 1984, Primary tissue cultures of human colon carcinomas in serum-free medium: An in vitro system for tumor analysis and therapy experiments, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. R. Liss, Inc., New York, (in press).

    Google Scholar 

  • Varner, H. H., Hewitt, A. T., and Martin, G. R., 1984, Methods for the isolation of chondronectin, in: Cell Culture Methods for Molecular and Cell Biology (D. Barnes, D. Sirbasku, and G. Sato), A. K. Liss, Inc., New York, (in press).

    Google Scholar 

  • Vartio, T., and Vaheri, A., 1981, A gelatin-binding 70,000-dalton glycoprotein synthesized distinctly from fibronectin by normal and malignant adherent cells. J Biol Chem. 256: 13085–13090.

    PubMed  CAS  Google Scholar 

  • Vlodavsky, I., and Gospodarowicz, D., 1981, Respective roles of laminin and fibronectin in adhesion of human carcinoma and sarcoma cells. Nature (London) 289: 304–306.

    Article  CAS  Google Scholar 

  • Vlodavsky, I., Lui, G. M., and Gospodarowicz, D., 1980, Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell 19: 607–616.

    Article  PubMed  CAS  Google Scholar 

  • Vuento, M., Salonen, E., Salminen, K., Pasanen, M., and Stenman, U., 1980, Immuno-chemical characterization of human plasma fibronectin. Biochem J. 191: 719–727.

    PubMed  CAS  Google Scholar 

  • Weinstein, R., Hoover, G. A., Stemerman, M. B., van der Spek, J., and Maciag, T., 1982, Fibronectin dependence for attachment in vitro: Smooth-muscle cell versus fibroblast, in: Growth of Cells in Hormonally Defined Media ( G. H. Sato, A. B. Pardee, and D. A. Sirbasku), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 145–154.

    Google Scholar 

  • Weiss, L., Poste, G., MacKearnin, A., and Willett, K., 1975, Growth of mammalian cells on substrates coated with cellular microexudates. J Cell Biol 64: 135–145.

    Article  PubMed  CAS  Google Scholar 

  • Weiss, R. E., and Reddi, A. H., 1980, Synthesis and localization of fibronectin during collagenous matrix-mesenchymal cell interaction and differentiation of cartilage and bone in vivo. Proc Natl Acad Sci USA. 77: 2074–2078.

    Article  PubMed  CAS  Google Scholar 

  • West, C. M., Lanza, R., Rosenbloom, J., Lowe, M., Holtzer, H., and Avdalovic, N., 1979, Fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts. Cell 17: 491–501.

    Article  PubMed  CAS  Google Scholar 

  • Wewer, U., Albrechtsen, R., and Ruoslahti, E., 1981, Laminin, a noncollagenous component of epithelial basement membrane synthesized by a rat yolk sac tumor. Cancer Res. 41: 1518–1524.

    PubMed  CAS  Google Scholar 

  • Whateley. J G., and Knox, P., 1980, Isolation of a serum component that stimulates the spreading of cells in culture. Biochem J. 185: 349–354.

    Google Scholar 

  • Wicha, M. S., Liotta, L. A., Garbisa, S., Kidwell, W. R., 1979, Basement membrane collagen requirements for attachment and growth of mammary epithelium. Exp Cell Res. 124: 181–190.

    Article  PubMed  CAS  Google Scholar 

  • Wicha, M. S., Liotta, L. A., Vonderhaar, B. K., and Kidwell, W. R., 1980, Effects of inhibition of basement membrane collagen deposition on rat mammary gland development. Dev Biol 80: 253–266.

    Article  PubMed  CAS  Google Scholar 

  • Wicha, M. S., Lowrie, G., Kohn, E., Baganandoss, P., and Mahn, T., 1982, Extracellular matrix promotes mammary epithelial growth and differentiation in vitro. Proc Natl Acad Sci USA. 79: 3213–3217.

    Article  PubMed  CAS  Google Scholar 

  • Yamada, K. M., and Olden, K., 1978, Fibronectins-adhesive glycoproteins of cell surface and blood. Nature (London) 275: 179–184.

    Article  CAS  Google Scholar 

  • Yamada, K. M., Kennedy, D. W., Kimata, K., and Pratt, R. M., 1980, Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. J Biol Chem. 255: 6055–6063.

    PubMed  CAS  Google Scholar 

  • Yamada, K. M., Kennedy, D. W., Grotendorst, G. R., and Momoi, T., 1981, Glycolipids: Receptors for Fibronectin? J Cell Physiol. 109: 343–351.

    Article  PubMed  CAS  Google Scholar 

  • Yamada, K. M., Kennedy, D. W., and Hayashi, M., 1982, Fibronectin in cell adhesion, differentiation and growth, in: Growth of Cells in Hormonally Defined Media ( G. H. Sato, A. B. Pardee, and D. A. Sirbasku), Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 131–143.

    Google Scholar 

  • Yang, J., Guzman, R., Richards, J., Jintoft, V., DeVault, M. R., Wellings, S. R., Nandi, S., 1980a, Primary culture of human mammary epithelial cells embedded in collagen gels. J Natl Cancer Inst. 65: 337–341.

    PubMed  CAS  Google Scholar 

  • Yang, J., Richards, J., Buzman, R., Imagawa, W., Nandi, S., 1980b, Sustained growth in primary culture of normal mammary epithelial cells imbedded in collagen gels. Proc Natl Acad Sci USA. 77: 2088–2092.

    Article  PubMed  CAS  Google Scholar 

  • Yang, N. S., Kirkland, W., Jorgensen, T., and Furmanski, P., 1980c, Absence of fibronectin and presence of plasminogen activator in both normal and malignant human mammary epithelial cells in culture. J Cell Biol 84: 120–130.

    Article  PubMed  CAS  Google Scholar 

  • Yaoi, Y., Onoda, T., Takahashi, H., 1972, Inhibition of mitosis in suspension culture of chick embryo cells. Nature (London) 237: 285–286.

    Article  CAS  Google Scholar 

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  1. Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA

    David Barnes

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  1. The Population Council, New York, New York, USA

    Jennie P. Mather

  2. The Rockefeller University, New York, New York, USA

    Jennie P. Mather

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Barnes, D. (1984). Attachment Factors in Cell Culture. In: Mather, J.P. (eds) Mammalian Cell Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9361-4_9

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