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Enzymatic Dispersion of Heart and Other Tissues

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Single-Channel Recording

Abstract

A large number of techniques have been developed for the enzymatic isolation of many types of cells since the first trials around 1970 (hepatocytes: Berry and Friend, 1969; cardiac myocytes: Vahouny et al., 1970; smooth muscle cells: Bagby et al., 1971). Most of the procedures have been designed to meet the needs of biochemical investigations. For electrophysiologists, enzymatic dissociation is of interest if the cells are not easily accessible otherwise or if they are coupled to a complex electrical network, as, for example, in heart. Enzymatically isolated cells also turned out to be very well suited for the patch clamp technique. They have a cell surface stripped of connective tissue, a prerequisite for the formation of a gigaseal between the current-recording pipette and the cell membrane (Neher, 1982).

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Authors and Affiliations

  1. II. Physiologisches Institut, Universität des Saarlandes, 6650, Homburg/Saar, Federal Republic of Germany

    G. Trube

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  1. G. Trube
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  1. Max-Planck-Institut für biophysikalische Chemie, Göttingen, Federal Republic of Germany

    Bert Sakmann  & Erwin Neher  & 

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© 1983 Plenum Press, New york

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Trube, G. (1983). Enzymatic Dispersion of Heart and Other Tissues. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7858-1_4

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  • DOI: https://doi.org/10.1007/978-1-4615-7858-1_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7860-4

  • Online ISBN: 978-1-4615-7858-1

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