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Bulk Separation of Neuronal Cell Bodies and Glial Cells in the Absence of Added Digestive Enzymes

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Research Methods in Neurochemistry

Abstract

Since the appearance of the procedure of Norton and Poduslo (1970), reviewed in detail in the first volume of this series, several new procedures for the bulk separation of neuronal cell bodies and glial cells have been reported in which all steps are carried out in the cold and no digestive enzyme(s) (Norton and Poduslo, 1970; Hemminki and Holmila, 1971; Giorgi, 1971; Kuenzle et al., 1972) is used to facilitate cell separation. In this chapter, we give a detailed description of these procedures and we also present a general survey of their applications in cellular neurochemistry.

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Author information

Author notes

  1. Julio M. Azcurra

    Present address: DTO Biologia, Facultad de Ciencias Exactas y Naturales, Casilla de Correo 1766, Buenos Aires, Argentina

Authors and Affiliations

  1. Laboratory of Neurochemistry, Mental Health Research Institute, University of Michigan Medical Center, Ann Arbor, Michigan, USA

    Otto Z. Sellinger & Julio M. Azcurra

Authors
  1. Otto Z. Sellinger
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  2. Julio M. Azcurra
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Editor information

Editors and Affiliations

  1. New York State Research Institute for Neurochemistry and Drug Addiction, Ward’s Island, New York, New York, USA

    Neville Marks

  2. Department of Biochemistry, Institute of Psychiatry, University of London, London, Great Britain

    Richard Rodnight

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© 1974 Plenum Press, New York

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Sellinger, O.Z., Azcurra, J.M. (1974). Bulk Separation of Neuronal Cell Bodies and Glial Cells in the Absence of Added Digestive Enzymes. In: Marks, N., Rodnight, R. (eds) Research Methods in Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7751-5_1

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  • DOI: https://doi.org/10.1007/978-1-4615-7751-5_1

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7753-9

  • Online ISBN: 978-1-4615-7751-5

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