Isolation of Brain Cell Nuclei

McEwen, Bruce S.; Zigmond, Richard E.

doi:10.1007/978-1-4615-7748-5_6

Bruce S. McEwen³ &
Richard E. Zigmond³

144 Accesses
20 Citations

Abstract

In choosing a method for subcellular fractionation, the investigator must first consider the particular question which he wishes to answer. In studies of the subcellular distribution of a substance of almost certain cytoplasmic localization, it is often sufficient to monitor (for purposes of estimating recoveries) the concentration of that substance in a crude “nuclear pellet” obtained by low-speed centrifugation of a sucrose homogenate. From most tissues, however, such a pellet is so heavily contaminated with cytoplasmic debris that it is totally unsatisfactory for situations in which any degree of nuclear localization is a serious possibility. For studies in which determination of nuclear localization of a substance is important, considerable effort must be given to the removal of cytoplasmic particles adhering to nuclei and to purifying the nuclei from such contamination.

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Authors and Affiliations

The Rockefeller University, New York, New York, USA
Bruce S. McEwen & Richard E. Zigmond

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Bruce S. McEwen
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Richard E. Zigmond
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New York State Research Institute, Neurochemistry and Drug Addiction, Ward’s lsland, New York, New York, USA
Neville Marks
Department of Biochemistry, Institute Psychiatry, University of London, London, Great Britain
Richard Rodnight

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McEwen, B.S., Zigmond, R.E. (1972). Isolation of Brain Cell Nuclei. In: Marks, N., Rodnight, R. (eds) Research Methods in Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7748-5_6

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DOI: https://doi.org/10.1007/978-1-4615-7748-5_6
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