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Production of Food Related Colorants by Culture of Plant Cells

The Case of Betalains

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Chemicals via Higher Plant Bioengineering

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 464))

Abstract

Betalains are vacuole pigments produced solely by Caryophyllales plants and few superior fungi. For many years, betalains have been used as food colorants. The aim of the present chapter is to present relevant aspects related to betalains characteristics and feasibility of their industrial production in bioreactors.

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References

  • Berlin, J.; Sieg, S.; Strack, D.; Bokern, M.; Harms, H. Production of betalains by suspension cultures of Chenopo-dium rubrum L. Plant Cell, Tiss. Org Cul. 1986, 5(2), 163–174

    Article  CAS  Google Scholar 

  • Bianco-Colomas, J.; Hugues, M.; Establishment and characterization of a betacyanin producing cell line of Amaranthus tricolor: inductive effects of light and cytokinin. J. Plant Physiol. 1990,136,734–739

    Article  CAS  Google Scholar 

  • Böhm, H.; Böhm, L.; Rink, E.; Establishment and characterization of a betaxanthin producing cell cultures from Portulaca grandiflora. Plant Cell Tiss. Org. Cult. 1991, 26, 75–82

    Article  Google Scholar 

  • Böhm, H.; Rink, E.; Betalains. Cap. 26, p.449–463. En “Cell culture and somatic cell genetics of plants”. Vol.5. Academic Press, N.Y., 1988

    Google Scholar 

  • Bokern, M.; Strack, D.; Synthesis of hydroxyccinamic acid esters of betacyanins via 1-0-acylglucosydes of hy-droxyccinamic acids by protein preparations from cell suspension cultures of Chenopodium rubrum and petalas of Lampranthus sociorum. Planta 1988,174, 101–105

    Article  CAS  Google Scholar 

  • Brodelius, P.; Pedersen, H.; Increasing secondary metabolites production in plant cell culture by redirecting transport. TIBTECH. 1993, 11, 30–36

    Article  CAS  Google Scholar 

  • Curtis, R.; Emery, A.; Plant cell suspension culture rheology. Biotechnol Bioeng. 1993,42, 520–526

    Article  PubMed  CAS  Google Scholar 

  • Dörnenburg, H.; and Knorr, D.; Cellular permeabilization of cultured plant tissue cultures by high electric field pulses or ultra high pressure for the recovery of secondary metabolites. Food Biotechnol 1993, 7 (1), 35–48

    Google Scholar 

  • Dörnenburg, H.; and Knorr, D.; Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb. Technol 1995,17, 674–684

    Article  Google Scholar 

  • Dörnenburg, H.; Knorr, D. Challenges and Opportunities of metabolite production from plant cell and tissue culture. Food Technol. 1997, 51(11), 47–54

    Google Scholar 

  • Dunlop, E.; Namdev. P.; Effect of fluid forces on plant cell suspension. Abstracts of Papers. 3rd International Conference on Bioreactor and Bioprocess Fluid Dynamics; Nienow, A. (Ed.); Mechanical Eng. Pub. Ltd., London, 1993; pp. 447–455

    Google Scholar 

  • Elliott, C. D.; The pathway of betalain biosynthesis: Effect of cytokinin on enzymatic oxidation and hydroxylation of tyrosine in Amaranthus tricolor seedlings. Physiol. Plant. 1983, 59, 428–437

    Article  CAS  Google Scholar 

  • Francis, J.; Food colorants today. World Ingred. 1994, Oct./Nov. 8-9, 11

    Google Scholar 

  • Gamborg, O. L.; Miller, R. A.; Ojima, K.; Nutrient requirements of suspension culture of soybean cells. Exp. Cell Res. 1968, 50, 151–156

    Article  PubMed  CAS  Google Scholar 

  • Girod, P.A.; Zryd, J.; Secondary metabolism in cultured red beet (Beta vulgaris) cells: differential regulation of betaxanthin and betacyanin biosynthesis. Plant Cell Tiss Org. Cult. 1991 25, 1–12

    Article  CAS  Google Scholar 

  • Goosen, M.; Large-scale insect cell culture. Current Opinion Biotechnol. 1992, 3: 99–104

    Article  CAS  Google Scholar 

  • Gullett, E.A.; Color and Food. In Encyclopedia of food science and Technology. Vol.1. (Ed) Y.H. Hui; John Wiley and Sons, New York, 1991; pp.452–460

    Google Scholar 

  • Havkin-Frenkel, D; Dorn, R.; Leustek, T.; Plant tissue culture for production of secondary metabolites. Food Technol 1997, 51(11), 56–59

    CAS  Google Scholar 

  • Hayward, P. M.; Colorantes (Food colorings). Tecnol Aliment. 1992,27(4-5), 34–43

    Google Scholar 

  • Hempel, J.; Böhm, H.; Betaxanthin pattern of hairy roots from Beta vulgaris var. Lutea and its alteration by feeding of amino acids. Phytochem. 1977, 44(5), 847–852

    Article  Google Scholar 

  • Heuer, S.; Strack, D.; Synthesis of betanin from betanidin and UDP-glucose by protein preparation from cell suspension cultures of Dorotheanthusbellidiformis (Burm, f). Planta, 1992,186: 626–628

    Article  CAS  Google Scholar 

  • Hirano, H.; Komamine, A.; Correlation of betacyanin synthesis with cell division in cell suspension cultures of Phytolacca americana. Physiol Plant, 1994, 90, 239–245

    Article  CAS  Google Scholar 

  • Hooker, B.; Lee, J.; An, G.; Response of plant tissue culture to high shear environment. Enzyme Microb. Technol 1989, 11, 484–490

    Article  CAS  Google Scholar 

  • Hooker, B.; Lee, J.; An, G.; Cultivation of plant cells in a stirred vessel: effect of impeller design. Biotechnol Bioeng. 1990, 35, 296–304

    Article  PubMed  CAS  Google Scholar 

  • Humphrey, A.; Plant cells as chemical factories: control and recovery of valuable products. Advances in Bioproc-ess Engineering; Galindo, E.; Ramirez T. (Eds.); Kluwer Academic Pub., 1994, 103–107

    Google Scholar 

  • Hunter, C. S.; Kilby, N. J.; Betanin production and release in vitro from suspension cultures of Beta vulgaris. Cap. 49.; Methods in molecular biology. Pollard, W.; Walker, J.M.; Humana Press. 1990, 545–554

    Google Scholar 

  • Ilker, R.; Invitro pigment production: an alternative to color synthesis. Food Technol. 1987, 41(4), 70–72

    CAS  Google Scholar 

  • Janes, D. A.; Thomas, N.; Callow, J.; Red beet batch culture demonstration of a bubble-free taylor-couette bioreac-tor. Biotechnol. Technol. 1987, 1(4), 257–262

    Article  Google Scholar 

  • Jiménez, A. A.; Dávila, O. G.; Villegas, G. T.; Del Villar, M. A.; Obtenci ón de colorantes de interes alimentario por cultivo de células de opuntia microdasys (Lehm) Pfeiff. Rev. Latinoamer. Quim. 1992, 23(1), 5–8

    Google Scholar 

  • Jiménez, A. A.; Gutiérrez, L. G.; Rodríguez, M. M.; Caracterizacion reológica e hidrodinámica del cultivo de células de betabel (B. vulgaris L.) productoras de betalainas en un biorreactor tipo tanque agitado. Propiedades físicas de los alimentos, Vol. 1; Hübinger, M.; Murr, F. X.; Aguilera, J. M. (Eds).; CYTED-UPV, Vlencia, Espana, 1996; 105–112

    Google Scholar 

  • Jiménez, A. A.; Rodríguez, M. M.; Gutiérrez, L. G. Unpublished data. 1998

    Google Scholar 

  • Joshi, J.; Elias, C.; Patole, M.; Role of hydrodynamic shear in the cultivation of animal, plant and microbial cells. Biochem. Eng. J. 1996, 62, 121–141

    CAS  Google Scholar 

  • Kato, A.; Kawazoe, S.; Soh, Y.; Viscosity of the broth of tobacco cells in suspension culture. J. Ferment. Technol. 1978, 56(3), 224–228

    Google Scholar 

  • Khlebnikov, A.; Dubois, B.; Kut, O. M.; Prenosil, J. E.; Growth and productivity of Beta vulgaris cell culture in fluidized bed reactors. Bioprocess Eng. 1995, 14,51–56

    Article  CAS  Google Scholar 

  • Kieran, P,; O’Donnell, H.; Malone, D.; MacLoughlin, P.; Fluid shear effects on suspension cultures of Morinda citrifolia. Biotechnol. Bioeng: 1995, 45, 415–425

    Article  PubMed  CAS  Google Scholar 

  • Kilby, N. J.; Hunter, C.S.; Repeated harvest of vacuole-located secondary product from in vitro grown plants cells using 1.02mhz ultrasound. Appl. Microbiol. Biotechnol. 1990, 33, 448–451

    Article  CAS  Google Scholar 

  • Kino-Oka, M.; Mine, K.; Taya, M.; Tone, S.; Bioreactor-based cultured of plant hairy roots for production and recovery of pigments. Biochem. Eng. 2001 1992a, 296–298

    Google Scholar 

  • Kino-Oka, M.; Hongo, Y.; Taya, M.; Tone, S.; Culture of red beet hairy root in bioreactor and recovery of pigment release by oxygen starvation. J. Chem. Eng. Jpn. 1992b, 25(5), 490–495

    Article  CAS  Google Scholar 

  • Kino-Oka, M.; Tone, S.; Extracellular production of pigment from red beet hairy roots accompanied by oxygen preservation. J. Chem. Eng. Jpn. 1996, 29(3), 488–493

    Article  CAS  Google Scholar 

  • Kishima, Y.; Nozaki, K.; Akasi, R.; Adachi, T.; Light inducible pigmentation in Portulaca callus; selection of high betalain producing cell line. Plant Cell Rep. 1991,10,304–307

    Article  CAS  Google Scholar 

  • Knorr, D.; Berlin, J.; Effects of immobilization and permeabilization procedures on growth of Chenopodium rubrum cells and amaranthin concentration. J. Food Sci. 1987, 52(5), 1397–1400

    Article  CAS  Google Scholar 

  • Knorr, D.; Beaumont, M.; Caster, C; Dörnemburg, H.; Gross, B.; Pandya, Y.; Romagnoli, L.-G.; Plant tissue culture for the production of naturally derive food ingredients. Food Technol. 1990, 44(6), 71–79

    CAS  Google Scholar 

  • Komamine, A.; Hirano, H.; Kagewata, K.; Suda, J.; Hirose, M.; Roy, R.; Sugiyama, M.; Sakuta, M.; Regulation mechanisms of biosynthesis of betacyanin and anthocyanin in relation to cell division activity in suspension cultures. Proceedings, Primary and Secondary Metabolism of Plants and Plant Cell Cultures, Schrip-sema, J.; Verpoorte, R. (Eds).; Leiden, The Netherlands, 1993, p. 105

    Google Scholar 

  • Leathers, R.; Davin, C; Zryd, J.; Betalain producing cell cultures of Beta vulgaris L. (Red beet). In Vitro Cell Dev. Biol. 1992, 28, 39–45

    Article  Google Scholar 

  • Mabry, T. J.; Taylor, A.; Turner, B. L.; The betacyanins and their distribution. Phytochem. 1963, 2, 61–64

    Article  CAS  Google Scholar 

  • Mabry, T. J.; Dreiding, A. S.; The betalaines. Recent advances in phytochemistry, Vol.1. Mabry, R.; Alston, E.; Runeckles, V. C. (Eds).; Appleton Century Crofts, New York, 1968; pp. 145–160

    Google Scholar 

  • Meijer, J.; ten Hoopen, H.; Luyben, K.; Libbenga, R.; Effects of hydrodynamic stress on cultured plant cells: A literature survey. Enzyme Microb. Technol. 1993, 15, 234–238

    Article  CAS  Google Scholar 

  • Meijer, J.; ten Hoopen, H.; Jan-Gameren, Y.; Luyben, K.; Libbenga, R.; Effects of hydrodynamic stress on the growth of plant cells in batch and continuous culture. Enzyme Microb. Technol. 1994, 16, 467–477

    Article  CAS  Google Scholar 

  • Metzner, A.; Otto, R.; Agitation of non-Newtonian fluids. A.I.Ch.E.J. 1957, 3(1), 3–9

    Article  CAS  Google Scholar 

  • Minale, L.; Piatelli, M.; De-Stefano, S.; Nicolaus, R. A.; Pigments of Centrospermae VI. Acylated betacyanins. Phytochem. 1966, 5, 1037–1052

    Article  CAS  Google Scholar 

  • Mueller, L. A.; Hinz, U.; Zryd, J. P.; The formation of betalamic acid and muscaflavin by recombinant DOPA-di-oxygenase from Amanita. Phytochem. 1997, 44(4),561–569

    Article  Google Scholar 

  • Mueller, L. A.; Hinz, U.; Uze, M.; Sautter, C.; Zryd, J. P. Complementation of betalain biosynthesis in P. grandiflora by a fungal DOPA-dioxigenase}. Experientia 1996, 52(2):A20

    Google Scholar 

  • Murashige, T.; Skoog, F.; A revised medium for rapid growth-promoting activity of various auxins. Plant Physiol. 1962,57,35–41

    Google Scholar 

  • Piatelli, M.; Minale, L.; Pigments of Centrospermae I. Betacyanins from Phyllocactus hybridus Hort. And Opuntia ficus-indica. Phytochem. 1964, 3, 307–311

    Article  Google Scholar 

  • Piatelli, M.; Betalains. Chemistry and biochemistry of plant pigments. Vol. 1. Goodwin, T.W. (Ed); Academic Press, New York, 1976; pp.560–596

    Google Scholar 

  • Piatelli, M.; The betalains: structure, biosynthesis and chemical taxonomy. The biochemistry of plants: A comprehensive treatise. Vol. 17. Secondary plant products. Conn, E.E. (Ed).; Academic Press, New York, 1981; pp.557–575

    Google Scholar 

  • Pras, N.; Bioconversion of naturally occurring precursors and related synthetic compounds using plant cell cultures. J. Biotechnol. 1992, 26, 29–62

    Article  PubMed  CAS  Google Scholar 

  • Rink, E.; Böhm, H. Changed betaxanthin pattern in violet flowers of Portulaca grandiflora after the feeding of DOPA. Phytochem. 1985, 24(7), 1475–1477

    Article  CAS  Google Scholar 

  • Rodríguez, M.; Jiménez, A.; Dávila, G.; Sepúlveda, G.; Effect of carbon source in cell suspension culture of Beta vulgaris. Biotechnol Lett. 1994, 16(8), 853–858

    Google Scholar 

  • Rodríguez, M M.; Jiménez, A. A.; Sepúlveda, J. G.; Development of an experimental model for the study of the biosynthesis of betalains (natural colorants) from tissue culture of Beta vulgaris. Papers 2nd International Symposium on Natural Colorants. Hereid, R C. (Ed).; S.I.C. Publishing Company, CT, 1996; pp. 409–416

    Google Scholar 

  • Rodríguez, M.; Galindo. E.; Efecto del estrés hidrodinámico sobre cultivos de células de Beta vulgaris. Abstracts of Papers, Proc. 2nd International Engineering Bioprocesses of Sociedad Mexicana de Biotecnología y Bioingenieria; Mazatlán, México, 1997; p. 155

    Google Scholar 

  • Rosendal-Jensen, S.; Juhl-Nielsen, B.; Dahlgren, R.; Use of chemistry en plant classification. Rev. Latinoamer.Quim. 1989, Suppl 1, 66–89.

    Google Scholar 

  • Sakuta, M.; Takagi, T.; Komamine, A.; Growth related accumulation of betacyanin in suspension cultures of Phy-tolacca americana L. J. Plant Physiol. 1986, 125, 337–343

    Article  CAS  Google Scholar 

  • Schenk, U. R.; Hildebrandt, C. A.; Medium and techniques for induction and growth of monocotyledoneus and di-cotyledoneus plan cell cultures. Can. J. Bot. 1972, 50, 199–204

    Article  CAS  Google Scholar 

  • Scragg, A.; Allan, E.; Leckie, F.; Effect of shear on the viability of plant cell suspension. Enzyme Microb. Technol. 1988, 10, 361–367

    Article  Google Scholar 

  • Scragg, A.; Large-scale plant cell culture: methods, applications and products. Current Opinion Biotechnol. 1992, 3, 105–109

    Article  CAS  Google Scholar 

  • Schwitzguébel, J. P.; Zryd, J. P.; Leathers, R.; From plant cells to biotechnology. Swiss Biotech. 1991, 9(1), 17–24

    Google Scholar 

  • Schwitzguébel, J. P; Biosynthesis of betalains by cultivation of red beetroot cells in bioreactors. Abstract at WEB site http://dgrwww.epfl.ch/GB/DP/JPS-l.html, 1997

    Google Scholar 

  • Sobkowska, E.; Czapski, L.; Kaczmarek, R.; Red table beet pigment as food colorant. Int. Food Ing. 1991, 3, 24–28

    Google Scholar 

  • Spears, K.; Developments in food colorings: the natural alternatives. TIBTECH 1988, 6 (8), 1973–1980

    Google Scholar 

  • Stafford, A.; The manufacture of food ingredients using plant cell and tissue cultures. Trends Food Sci. Technol. 1991, 5(2), 116–122

    Article  Google Scholar 

  • Steghis, W.; Strack, D.; Betalains. The alkaloids. Vol. 39; Brossi, A. (Ed.); Academic Press, Orlando, FL, 1991; pp. 1–62

    Google Scholar 

  • Strack, D.; Steglich, W.; Wray, V; Betalains. Methods in plant biochemistry. Vol. 8; Academic Press, Orlando, FL, 1993; pp.421–450

    Google Scholar 

  • Takeda, T.; Seki, M.; Furusaki, S.; Hydrodynamic damage of cultured cells of Carthamus tinctorius in a stirred tank reactor. J. Chem. Eng. 1994, 27(4), 466–471

    Article  CAS  Google Scholar 

  • Tan, W.; Dai, G.; Chen, Y.; Quantitative investigations of cell-bubble interactions using a foam fractionation technique. Cytotechnology 1994, 15 (1-3), 321–328

    Google Scholar 

  • Tanaka, H.; Technological problems in cultivation of plant cells at high density. Biotechnol. Bioeng. 1988, 23, 1203–1218

    Article  Google Scholar 

  • Tanaka, H.; Semba, H.; Jitsufuchi, T.; Harada, H.; The effect of physical stress on plant cells in suspension cultures. Biotechnol Lett. 1988, 10(7), 485–490

    Article  Google Scholar 

  • Taticek R.; Moo-Young, M.; Legge, L.; The scale-up of plant cell culture: engineering considerations. Plant Cell Tiss.Cult. 1994, 24, 139–158

    Article  Google Scholar 

  • Thomas, C.; Problems of shear in biotechnology. Chemical engineering problems in biotechnology. Winkler, M. (Ed.); Elsevier, Sci. Pub. England, 1990; pp. 25–93

    Google Scholar 

  • Tramper, J.; Gooijer, D.; Vlak, J.; Scale-up considerations and bioreactor development for animal cell cultivation. Insect cell culture engineering. Goosen, M.; Daugulis, A.; Faulkner, P. (Eds.); Marcel Dekker, Inc., New York, 1993; pp. 139–177

    Google Scholar 

  • Trejo, T. G.; Rodríguez, M. M.; Jiménez, A. A.; De Jesus, S. A.; Martinez, B. B.; Sepúlveda, J. G.; Salcedo, M. G.; Estudios para 1a producci ón de betalainas en cultivos de Beta vulgaris: Efecto de la fuente de carbono y nitrógeno. Abstracts of Papers, Proc. 2nd International Engineering Bioprocesses of Sociedad Mexicana de Biotecnología y Bioingenieria; Mazatlán, México, 1997; p.206

    Google Scholar 

  • Trezzini, G. F.; Zryd, J. P.; Portulaca grandiflora: A model system for the study of the biochemistry and genetics of betalain synthesis. Acta Hort. 1990, 280, 581–585

    Google Scholar 

  • Trezzini, G. F.; Zryd, J. P.; Two betalains from Portulaca grandiflora. Phytochem. 1991a, 30(6), 1897–1899

    Article  CAS  Google Scholar 

  • Trezzini, G. F.; Zryd, J. P.; Characterization of some natural and semi-synthetic betaxantins. Phytochem, 1991b, 30(6), 1901–1903

    Google Scholar 

  • Wagner, F.; Vogelman, H.; Cultivation of plant tissue cultures in bioreactors and formation of secondary metabolites. Plant tissue culture and its biotechnological application, Barz, W.; Reinhard, R.; Zenk, H. (Eds.), Springer-Verlag, Berlin, 1977; pp. 245–255

    Chapter  Google Scholar 

  • Weller, A. T.; Lasure, L. L.; Betalains in beet root tissue culture. J. Food Sci. 1982, 47,162–163

    Article  Google Scholar 

  • von-Elbe, J. H.; The betalaines. Current aspects of food colorants, Chap. 3, Furia, E.T. (Ed).; CRC Press Inc., Boca Raton, FL, 1977; pp. 29–39

    Google Scholar 

  • Zhong, J.; Seki, T.; Kinoshita, S.; Yoshida, T.; Rheological characteristics of cell suspension and cell culture of Periella frutescens. Biotechnol. Bioeng. 1990, 40,1256–1262.

    Article  Google Scholar 

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Jiménez-Aparicio, A., Gutiérrez-López, G. (1999). Production of Food Related Colorants by Culture of Plant Cells. In: Shahidi, F., Kolodziejczyk, P., Whitaker, J.R., Munguia, A.L., Fuller, G. (eds) Chemicals via Higher Plant Bioengineering. Advances in Experimental Medicine and Biology, vol 464. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4729-7_15

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