Abstract
Red-violet cell suspension cultures of Chenopodium rubrum were found to accumulate the betacyanins amaranthin, celosianin and betanin and the betaxanthins vulgaxanthin I and vulgaxanthin II. Under a 16-h daylight regime the cells accumulated 0.3–0.4% betacyanins on a dry mass basis after 2–3 weeks of cultivation on the growth medium. Experiments to define a production medium for betacyanins failed with this habituated line. The accumulation could however be increased up to 1% or 100 mg betacyanins/1 by feeding tyrosine and by adaptation of the inoculum size to the nutrient concentration.
Similar content being viewed by others
References
Adams JP, von Elbe JH, Amundson CH (1976) Production of betacyanin concentrate by fermentation of red beet juice with Candida utilis. J Food Sci 41: 78–81
Berlin J, Forche E, Wray V, Hammer J, Hösel W (1983) Formation of benzophenanthridine alkaloids by suspension cultures of Eschscholtzia californica. Z Naturforsch 38c: 346–352
Burlager T, Sasse F, Berlin J (1984) Anthraquinones in cell suspension cultures of Rhamnus frangula. Farm-Tijdschrift België 61: 331
Colijn CM, Johnson LMV, Schram AW, Kool AJ (1981) Synthesis of malvidin and petunidin in pigmented tissue cultures of Petunia hybrida. Protoplasma 107, 63–68
Constabel F, Nassif-Makki H (1971) Betalainbildung in Beta-Calluskulturen. Ber Dtsch Bot Ges 84: 629–636
Dougall DK, Johnson JM, Whitten GH (1980) A clonal analysis of anthocyanin accumulation by cell cultures of wild carrot. Plant 149: 292–297
Elliot CD (1983) The pathway of betalain biosynthesis: Effect of cytokinin on enzyme oxidation and hydroxylation of tyrosine in Amaranthus tricolor seedlings. Physiol Plant 59: 428–437
Endress R (1976) Betacyan-Akkumulation in Kallus von Portulacca grandiflora unter dem Einfluß von Phytohormonen und Cu2+-Ionen auf unterschiedlichen Grundmedien. Biochem Physiol Pflanzen 19: 87–98
Harms H, Dehnen W, Mönch W (1977) Benzo(a)pyrene metabolites formed by plant cells. Z Naturforsch 32c: 321–326
Heller W, Egin-Bühler B, Gardiner S, Knobloch K-H, Matern U, Ebel J, Hahlbrock K (1979) Enzymes of general phenylpropanoid metabolism and of flavonoid glucoside biosynthesis. Plant Physiol 64: 371–373
Hermann K, Dreiding AS (1977) Totalsynthese von Betalainen. Helv Chim Acta 60: 673–683
Knobloch K-H, Berlin J (1981) Effects of media constituents on the formation of secondary products in cell suspension cultures of Catharanthus roseus. In: MooYoung M (ed) Advances in Biotechnology. Toronto: Pergamon Press, Vol. 1, pp 129–133
Kubek DJ, Schuler ML (1980) The effect of variations in carbon and nitrogen concentrations on phenolics formation in plant cell suspension cultures. Lloydia 43: 87–92
Mabry TJ (1980) Betalains. In: Pirson A, Zimmerman MH (eds) Encyclopedia of Plant Physiology, Berlin/Heidelberg/New York: Springer-Verlag, pp 513–533
Misawa M (1980) Industrial and governmental research. In: Staba EJ (ed) Plant Tissue Culture as a Source of Biochemicals. Boca Raton, Florida: CRC Press, pp 167–190
Piatelli M (1981) The betalains: structure, biosynthesis and chemical taxonomy. In: Conn EE (ed) The Biochemistry of Plants. New York: Academic Press, Vol. 7, pp 557–575
Piatelli M, de Nicola MG, Castrogiovanni V (1969) Photocontrol of amaranthin synthesis in Amaranthus tricolor. Phytochemistry 8: 731–736
Pourrat H, Lejeune B, Regerat F, Pourrat A (1983) Purification of red beet root dye by fermentation. Biotechnol Lett 5: 381–384
Sasse F, Heckenberg U, Berlin J (1982) Accumulation of β-carboline alkaloids and serotonin by cell cultures of Peganum harmala. I. Correlation between plants and cell cultures and influence of media constituents. Plant Physiol. 69: 400–404
Schiel O, Jarchow-Redecker K, Piehl GW, Lehmann J, Berlin J (1984) Increased formation of cinnamoyl putrescines by fedbatch fermentation of cell suspension cultures of Nicotiana tabacum. Plant Cell Rep. 3: 18–20
Schwartz SJ, von Elbe JH (1980) Quantitative determination of betacyanin pigments by high performance liquid chromatography. J Agric Food Chem 28: 540–543
Sieg S (1983) Betalainbildung bei einer Chenopodium rubrum Zellkultur. Diplom Thesis, Technical University Braunschweig, FRG
Strack D, Engel U, Reznik H (1981) High performance liquid chromatography of betalains and its application to pigment analysis of Aizoaceae and Cactaceae. Z Pflanzenphysiol 101: 215–222
Strack D, Bokern M, Berlin J, Sieg S (1984) Metabolic activity of hydroxycinnamic acid glucose esters in cell suspension cultures of Chenopodium rubrum. Z Naturforsch 39c: 902–907
Vincent KR, Scholz RG (1978) Separation and quantification of red beet betacyanins and betaxanthins by high-performance liquid chromatography. J Agric Food Chem 26: 812–816
Yamamoto Y, Mizuguchi R, Yamada Y (1982) Selection of a high and stable pigment producing strain of cultured Euphorbia millii cells. Theor Appl Gen 61: 113–116
Zenk MH (1980) Enzymatic synthesis of ajmalicine and related indole alkaloids. J Nat Prod (Lloydia) 43: 438–451
Zryd JP, Bauer J, Wyler H, Lavanchy P (1982) Pigment biosynthesis and precursor metabolism in red beet semi-continuous cell suspension cultures. In: Fujiwara A (ed) Plant Tissue Culture 1982. Tokyo: Maruzen Co., pp 387–388
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berlin, J., Sieg, S., Strack, D. et al. Production of betalains by suspension cultures of Chenopodium rubrum L.. Plant Cell Tiss Organ Cult 5, 163–174 (1986). https://doi.org/10.1007/BF00040126
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040126