Abstract
Brain microvessel endothelial cells (BMEC) form a selectively permeable barrier known as the blood-brain barrier (BBB) (16). The barrier is maintained structurally by the formation of tight junctions between adjacent BMEC plasma membranes (1). As a result, metabolites exchanged between the blood and brain must pass through the BMEC and BMEC, are marked by transport systems that are highly expressed in comparison to endothelial cells of non-barrier origin.
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References
Brightman, M.W., and Reese, T.S., Junctions between intimately apposed cell membranes in the vertebrate brain, J. Cell Biol. 40: 638–677, 1969.
Crone, C., Facilitated transfer of glucose from blood into brain tissue, J. Physiol. London 181: 103–113, 1965.
Gay, D., and Esiri, M., Blood-brain barrier damage in acute multiple sclerosis plaques. An immunological study, Brain 114: 557–572, 1991.
Gerhart, D.Z., Broderius, M.A., and Drewes, L.R., Cultured human and canine endothelial cells from brain microvessels, Brain Res. Bull. 21: 785–793, 1988.
Gerhart, D.Z., LeVasseur, R.J., Broderius, M.A., and Drewes, L.R., Glucose transporter localization in brain using light and electron immunocytochemistry, J. Neurosci. Res. 22: 464–472, 1989.
Guerin, C., Laterra, J., Drewes, L.R., Brem, H., and Goldstein, G.W., Vascular expression of glucose transporter in experimental brain neoplasms, Am.J. Path. 140: 417–425, 1992.
Guerin, C., Laterra, J., Hruban, R., Brem, H., Drewes, L.R., and Goldstein, G.W., The glucose transporter and blood-brain barrier of human brain tumors, Ann. Neurol. 28: 758–765, 1990.
Hasegawa, H., Ushio, Y., Hayakawa, T., Yamada, K., and Mogami, H., Changes of the blood-brain barrier in experimental metastatic brain tumors, J. Neurosurg. 59: 304–310, 1983.
Joó, F., The cerebral microvessels in culture, an update, J. Neurochem. 58: 1–17, 1992.
Kalaria, R.N., and Harik, S.I., Reduced glucose transporter at the blood-brain barrier and in cerebral cortex in Alzheimer disease, J. Neurochem. 53: 1083–1088, 1989.
Kalaria, R.N., Gravina, S.A., Schmidley, J.W., Perry, G., and Harik, S.I., The glucose transporter of the human brain and blood-brain barrier, Ann. Neurol. 24: 757–764, 1988.
Madri, J.A., Dreyer, B., Pitlick, F.A., and Furthmayr, H., The collagenous components of the subendothelium. Correlation of structure and function, Lab. Invest. 43: 303–315, 1980.
Meyer, J., Mischeck, U., Vehyl, M., Henzel, K., and Galla, H.-J., Blood-brain barrier characteristic enzymatic properties in cultured brain capillary endothelial cells, Brain Res. 514: 305–309, 1990.
Mooradian, A.D., Morin, A.M., Cipp, L.J., and Haspel, H.C., Glucose transport is reduced in the blood-brain barrier of aged rats, Brain Res. 551: 145–149, 1991.
Pardridge, W.M., Boado, R.J., and Farrell, C.R., Brain-type glucose transporter (Glut-1) is selectively localized to the blood-brain barrier. Studies with quantitative western blotting and in situ hybridization, J. Biol. Chem. 265: 18035–18040, 1990.
Reese, T.S., and Karnovsky, M.J., Fine structural localization of a blood-brain barrier to exogenous peroxidase, J. Cell Biol. 34: 207–217, 1967.
Rhodes, R.H., Evidence of serum-protein leakage across the blood-brain barrier in the acquired immunodeficiency syndrome, J. Neuropathol. Exp. Neurol. 50: 171–183, 1991.
Voyta, J.C., Via, D.P., Butterfield, C.E., and Zetter, B.R., Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein, J. Cell Biol. 99: 2034–2040, 1984.
Weiler-Guttler, H., Zinke, H., Mockel, B., Frey, A., and Gassen, H.G., cDNA cloning and sequence analysis of the glucose transporter from porcine blood-brain barrier, Biol. Chem. Hoppe-Seyler 370: 467–473, 1989.
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Hemmila, J.M., Drewes, L.R. (1993). Glucose Transporter (Glut1) Expression by Canine Brain Microvessel Endothelial Cells in Culture: An Immunocytochemical Study. In: Drewes, L.R., Betz, A.L. (eds) Frontiers in Cerebral Vascular Biology. Advances in Experimental Medicine and Biology, vol 331. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2920-0_3
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DOI: https://doi.org/10.1007/978-1-4615-2920-0_3
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