Abstract
Cells from the established human line NHIK** 3025 were labelled with hematoporphyrin derivative In vitro. Subsequently, the cells were treated with light and hyperthermia. The cells could be irradiated either before, during or after the incubation at a hyperthermic temperature. It was shown that hyperthermia given shortly after the light exposure gave a synergistic killing effect. In spite of some loss of porphyrins from the cells, the light sensitivity increased 20 min after a light irradiation. At later times, the cells apparently repaired some of the photodynamic damage at 37°C. At higher temperatures, the repair was inhibited.
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Abbreviations
- PDT:
-
photodynamic therapy
- Hpd:
-
hematoporphyrin derivative
- NHIK:
-
Norsk Hydro’s Institute for Cancer Research
- MEM:
-
minimal essential medium
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© 1985 Plenum Press, New York
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Christensen, T., Smedshammer, L., Wahl, A., Moan, J. (1985). Photodynamic Effects And Hyperthermia In Vitro. In: Kessel, D. (eds) Methods in Porphyrin Photosensitization. Advances in Experimental Medicine and Biology, vol 193. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2165-1_8
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DOI: https://doi.org/10.1007/978-1-4613-2165-1_8
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