Abstract
The polymerase chain reaction (PCR) benefits from rapid temperature cycling (Wittwer et al., 1994). In particular, rapid cycling appears to improve the quantitative PCR of rare transcripts (Tan and Weis, 1992). The glass capillaries used as sample containers for rapid cycling are natural cuvettes for fluorescence analysis. Fluorometric monitoring of PCR has been reported with double-stranded DNA (dsDNA) dyes (Higuchi et al., 1992; Higuchi et al., 1993; Ishiguro et al., 1995; Wittwer et al., 1997a) and sequence-specific probes (Lee et al., 1993; Livak et al., 1995; Wittwer et al., 1997a). We have integrated a fluorimeter with a rapid temperature cycler for fluorescence monitoring during amplification (Wittwer et al., 1997b). Both cycle-by-cycle fluorescence monitoring and continuous (within cycle) monitoring offer unique quantitative information.
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© 1998 Birkhäuser Boston
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Wittwer, C., Ririe, K., Rasmussen, R. (1998). Fluorescence Monitoring of Rapid Cycle PCR for Quantification. In: Ferré, F. (eds) Gene Quantification. Advanced Biomedical Technologies. Birkhäuser Boston. https://doi.org/10.1007/978-1-4612-4164-5_8
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DOI: https://doi.org/10.1007/978-1-4612-4164-5_8
Publisher Name: Birkhäuser Boston
Print ISBN: 978-1-4612-8682-0
Online ISBN: 978-1-4612-4164-5
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