Abstract
DNA amplification requires temperature cycling of the sample. From the viewpoint of the sample, the only relevant characteristics of a temperature cycler are its speed and homogeneity. How fast the sample temperature can be changed largely determines the cycle time. How uniform the sample temperature is affects reproducibility. As cycle speed increases, it becomes harder to maintain homogeneous temperatures within and between samples. Standard commercial instrumentation usually completes 30 cycles (94, 55, 74°C) in about 2–4 hr. A new “high-performance” system requires about half as much time and is reported to run two temperature profiles (60, 94 °C) in a little over an hour (Haff et al., 1991). “Rapid cycle DNA amplification” as used here refers to completion of 30 cycles of amplification in 10–30 min.
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© 1994 Springer Science+Business Media New York
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Wittwer, C.T., Reed, G.B., Ririe, K.M. (1994). Rapid Cycle DNA Amplification. In: Mullis, K.B., Ferré, F., Gibbs, R.A. (eds) The Polymerase Chain Reaction. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0257-8_15
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DOI: https://doi.org/10.1007/978-1-4612-0257-8_15
Publisher Name: Birkhäuser, Boston, MA
Print ISBN: 978-0-8176-3750-7
Online ISBN: 978-1-4612-0257-8
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