Summary
Wound healing and granulation tissue formation can be accelerated by inoculation with live pathogenic microorganisms such as Staphylococcus aureus. To study the effects of Staphylococcus aureus bacteria and its products on granulation tissue formation further, hollow cylindrical viscose cellulose sponges were implanted subcutaneously as an inductive matrix for the growth of granulation tissue. The experimental implants were injected immediately after implantation with saline solution containing the bacteria or the product under study. The control implants were treated correspondingly with saline solution. Analyses of wound fluid and granulation tissue were carried out one or two weeks after implantation. Implants inoculated with 1 × 105 organisms developed infection with pus formation while implants contaminated with 100 organisms showed no infection. In wound fluid specimens collected from the infected implants correlation between the number of polymorphonuclear leukocytes and prostaglandin E2 concentration was statistically significant. The most prominent finding in contaminated but uninfected implants was an enhanced local blood flow. After daily application of 10 ng of Staphylococcal enterotoxin A (SEA), statistically significant increase was observed in the accumulation of collagen hydroxyproline (+70%). The response was dose-related and the threshold dose was close to 1 ng. The mean amounts of DNA in SEA-treated groups, reflecting the number of cells in repair tissue, remained close to the control group. SEA only, but not staphylococcal enterotoxins B, C1, C2, D, E or TSST-1 in daily doses of 100 ng was effective. To conclude, these findings may explain some of the mechanisms leading to Staphylococcus aureus-induced acceleration of wound healing.
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Laato, M., Niinikoski, J., Gerdin, B. (1990). The Effect of Staphylococcus aureus Bacteria and its Products on Wound Healing. In: Wadström, T., Eliasson, I., Holder, I., Ljungh, Å. (eds) Pathogenesis of Wound and Biomaterial-Associated Infections. Springer, London. https://doi.org/10.1007/978-1-4471-3454-1_3
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DOI: https://doi.org/10.1007/978-1-4471-3454-1_3
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