Abstract
Radish, Raphanus sativus L., is cultivated globally and exists as two broad categories according to the size of the swollen hypocotyl and taproot. Throughout temperate regions, a small-rooted, short-season radish is cultivated exclusively as a salad crop. Such radishes differ in shape (elongated to flattened spheres) and skin colour (white or red), with the main economic varieties exhibiting a spherical root with red skin and white flesh. The other major group of radish, the large-rooted types, are widely grown in the Far East and are adapted to both temperate and tropical conditions and exhibit a greater diversity of phenotype compared to Western varieties in terms of colour of skin (green, yellow, purple and black) and flesh (white, red, purple and green). Two other minor forms of radish are also cultivated, but not as a root crop. Mougri-radish (rat-tail) is mainly grown in south-east Asia for its edible leaves and very long (80 cm in length) immature seed pods. Fodder radish, is also cultivated for its foliage but is used as fodder or green manure in south-east Asia and parts of Western Europe.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Crisp P (1995). Radish. In: Smartt J, Simmonds NW (eds.), Evolution of Crop Plants, 2nd edn., Longman Group UK, Harlow, UK pp. 86–89.
Chinese Agricultural Press (2000). (ed.) Ministry of Agriculture of the Peoples Republic of China.
Annual Agricultural Reports of Korea (1998).
Japanese Governmental Figures Report (2000).
Watt JM and Breyer-Brandwijk MG (1962). In: The Medicinal and Poisonous Plants of Southern and Eastern Africa, 2nd edn., E. & S. Livingstone, Edinburgh and London.
Curtis IS (2003). The noble radish: past, present and future. Trends in Plant Science, 8: 305–307.
Wang L, Wei L, Wang L and Xu C (2002). Effects of peroxidase on hyperlipidemia in mice. Journal of Agricultural Food Chemistry, 50: 868–870.
Nakamura Y, Iwahashi T, Tanaka A, Koutani J, Matsuo T, Okamoto S, Sato K and Ohtsuki K (2001). 4-(Methylthio)-3-butenyl isothiocyanate, a principal antimutagen in daikon (Raphanus sativus; Japanese white radish). Journal of Agricultural Food Chemistry, 49: 5755–5760.
Banga O and Van Bennekom JL (1962). Breeding radish for winter production under glass. Euphytica, 11: 311–326.
Hida K and Ashizawa M (1985). Breeding of radishes for Fusarium resistance. Japan Agricutural Research Quarterly, 19: 190–195.
Williams PH and Pound GS (1963). Nature and inheritance of resistance to Albugo candida in radish. Phytopathology, 53: 1150–1154.
Shimizu S, Kanazawa K, Kono H and Yokota Y (1963). Studies on breeding radish for resistance to virus. Bulletin of the Horticultural Research Station. Series A, Hiratsuka, 2: 83–106.
Ogura H (1968). Studies on the new male sterility in Japanese radish with special reference to utilization of this sterility toward the practical raising of hybrid seeds. Memoirs of the Faculty of Agriculture, Kagoshima University, 6: 39–78.
Bonhomme S, Budar F, Lancelin D, Small I, Defrance MC and Pelletier G (1992). Sequence and transcript analysis of the Nco25 Ogura-specific fragment correlated with cytoplasmic male sterilty in Brassica cybrids. Molecular and General Genetics, 235: 340–348.
Yamagishi H and Terachi T (1997). Molecular and biological studies on male-sterile cytoplasm in the Cruciferae. IV. Ogura-type cytoplasm found in the wild radish, Raphanus raphanistrum. Plant Breeding, 116: 323–329.
Lee S-S (1987). Bolting in radish. In: Asian and Pacific Council (eds.), Improved Vegetable production in Asia.(pp. 60–70) Food Fertilizer Technology Center, Book series no. 36, Taipei, Taiwan, Republic of China.
Matsubara S and Hegazi HH (1990). Plant regeneration from hypocotyl callus of radish. HortScience, 25: 1286–1288.
Jeong WJ, Min SR and Liu JR (1995). Somatic embryogenesis and plant regeneration in tissue cultures of radish (Raphanus sativus L.). Plant Cell Reports, 14: 648–651.
Lichter R (1989). Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species. Plant Breeding, 103: 119–123.
Takahata Y, Komatsu H and Kaizuma N (1996). Microspore culture of radish (Raphanus sativus L.): influence of genotype and culture conditions on embryogenesis. Plant Cell Reports, 16: 163–166.
Pua E-C and Lee JEE (1995). Enhanced de novo shoot morphogenesis in vitro by expression of antisense 1-aminocyclopropane- 1 -carboxylate oxidase gene in transgenic mustard plants. Planta, 196: 69–76.
Chi G-L, Lin W-S, Lee JEE and Pua E-C (1994). Role of polyamines on de novo shoot morphogenesis from cotyledons of Brassica campestris ssp. pekinensis (Lour) Olsson in vitro Plant Cell Reports, 13: 323–329.
Pua E-C, Sim G-E, Chi G-L and Kong L-F (1996). Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey)in vitro. Plant Cell Reports, 15: 685–690.
Curtis IS, Nam HG and Sakamoto K (2004). Optimized shoot regeneration system for the commercial Korean radish ‘Jin Ju Dae Pyong’. Plant Cell, Tissue and Organ Culture, 77: 81–87.
Murashige T and Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473–497.
Lazo GR, Stein PA and Ludwigb RA (1991). A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Bio/Technology, 9: 963–967.
Clough SJ and Bent AF (1998). Floral-dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal, 16: 735–743.
Qing CM, Fan L, Lei Y, Bouchez D, Tourneur C, Yan L and Robaglia C (2000). Transformation of pakchoi (Brassica rapa L. ssp. chinensis) by Agrobacterium infiltration. Molecular Breeding, 6: 67–72.
Trieu AT, Burleigh SH, Kardailsky IV, Maldonado-Mendoza IE, Versaw WK, Blaylock LA, Shin H, Chiou T-J et al. (2000). Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. The Plant Journal, 22: 531–541.
Curtis IS and Nam HG (2001). Transgenic radish (Raphanus sativus L. var. longipinnatus Bailey) by floral-dip method — plant development and surfactant are important in optimizing transformation efficiency. Transgenic Research, 10: 363–371.
Curtis IS, Nam HG, Yun JY and Seo KH (2002). Expression of an antisense GIGANTEA (GI) gene fragment in transgenic radish causes delayed bolting and flowering. Transgenic Research, 11: 249–256.
Dellaporta SL, Wood J and Hicks IB (1983). A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1: 19–21.
Chomczynski P and Mackey K (1995). Modification of the TRI ReagentTM procedure for isolation of RNA from polysaccharide — and proteoglycan — rich sources. Biotechniques, 19: 942–945.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Kluwer Academic Publishers
About this chapter
Cite this chapter
Curtis, I.S. (2004). Genetic Transformation of Radish (Raphanus Sativus L.) by Floral-Dipping. In: Curtis, I.S. (eds) Transgenic Crops of the World. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2333-0_20
Download citation
DOI: https://doi.org/10.1007/978-1-4020-2333-0_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-015-7021-3
Online ISBN: 978-1-4020-2333-0
eBook Packages: Springer Book Archive