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Protein–Protein Interactions in Abiotic Stress Signaling: An Overview of Biochemical and Biophysical Methods of Characterization

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Plant Abiotic Stress Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2642))

Abstract

The identification and characterization of bona fide abiotic stress signaling proteins can occur at different levels of the complete in vivo signaling cascade or network. Knowledge of a particular abiotic stress signaling protein could theoretically lead to the characterization of complete networks through the analysis of unknown proteins that interact with the previously known protein. Such signaling proteins of interest can indeed be experimentally used as bait proteins to catch interacting prey proteins, provided that the association of bait proteins and prey proteins should yield a biochemical or biophysical signal that can be detected. To this end, several biochemical and biophysical techniques are available to provide experimental evidence for specific protein–protein interactions, such as co-immunoprecipitation, bimolecular fluorescence complementation, tandem affinity purification coupled to mass spectrometry, yeast two hybrid, protein microarrays, Förster resonance energy transfer, or fluorescence correlation spectroscopy. This array of methods can be implemented to establish the biochemical reality of putative protein–protein interactions between two proteins of interest or to identify previously unknown partners related to an initially known protein of interest. The ultimate validity of these methods however depends on the in vitro/in vivo nature of the approach and on the heterologous/homologous context of the analysis. This chapter will review the application and success of some classical methods of protein–protein interaction analysis in the field of plant abiotic stress signaling.

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Authors and Affiliations

  1. UMR 6553 ECOBIO (Ecosystems-Biodiversity-Evolution), CNRS, Université de Rennes, Brittany, France

    Ivan Couée & Gwenola Gouesbet

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  1. Ivan Couée
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  2. Gwenola Gouesbet
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Correspondence to Ivan Couée .

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  1. UMR 6553 ECOBIO (Ecosystems-Biodiversity-Evolution), Centre National de la Recherche Scientifique (CNRS), University of Rennes, Rennes, France

    Ivan Couée

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Couée, I., Gouesbet, G. (2023). Protein–Protein Interactions in Abiotic Stress Signaling: An Overview of Biochemical and Biophysical Methods of Characterization. In: Couée, I. (eds) Plant Abiotic Stress Signaling. Methods in Molecular Biology, vol 2642. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3044-0_17

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  • DOI: https://doi.org/10.1007/978-1-0716-3044-0_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3043-3

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