Abstract
During the last decades, signaling pathways responsible for the initiation of gastrulation in mammalian embryos have been identified. However, the physical rules governing the tissue spatial patterning and the extensive morphogenetic movements occurring during that process are still elusive. Progress on these issues is slowed by the difficulty to record or perturb the patterning events in real time, especially in mammalian embryos that develop in utero. Because they permit easy observation and manipulation, in vitro model systems offer an exciting opportunity to dissect the rules governing the organization of the mammalian gastrula. For instance, it is sufficient to cultivate human embryonic stem cells on micropatterned substrates to reveal their self-organization potential. We present here a method to obtain micropatterned mouse Epiblast Like Cells colonies, providing a convenient way to compare spatial organization of mouse and human pluripotent stem cells and to complement the characterization of mutant embryos in a controlled environment.
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Acknowledgements
This work was supported by French Agence Nationale de la Recherche (ANR-15-CE13-0007), Human Frontier Science Program (HFSP CDA00063/2015-C), and Institut National du Cancer (INCa 2014-1-PL BIO-01-UP 7-1). We are grateful to all members of the groups of J. Collignon (IJM) and P. Hersen (Institut Curie) for fruitful discussions during this project.
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Simon, G., Plouhinec, JL., Sorre, B. (2022). Differentiation of EpiLCs on Micropatterned Substrates Generated by Micro-Contact Printing. In: Osteil, P. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 2490. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2281-0_18
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DOI: https://doi.org/10.1007/978-1-0716-2281-0_18
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