Abstract
Direct infusion or “shotgun” mass spectrometry provides a fast strategy to measure different classes of lipids, combining rapid analysis and short idle time. In contrast to liquid chromatography–mass spectrometry (LC-MS), the lipids are infused into the mass spectrometer without prior separation by liquid chromatography. Ions are separated in the quadrupole of a tandem mass spectrometer, and after collision-induced dissociation fragments are quantified relative to internal standards in the third quadrupole or in the time-of-flight mass analyzer of a triple quadrupole or quadrupole time of flight (Q-TOF) mass spectrometer. Abundant lipids, that is, galactolipids and phospholipids in leaves, are measured in crude lipid extracts, while less abundant lipids can be measured after enrichment by solid-phase extraction. Here we describe protocols for the quantification of the major plant glycerolipids (galactolipids, phospholipids, diacylglycerol, and triacylglycerol) using nanospray direct infusion mass spectrometry. This provides a strategy for comprehensive, highly sensitive, high-throughput lipidomic analyses.
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Acknowledgments
We would like to thank the DFG for funding of MS equipment (Forschungsgroßgeräte nach Art. 91b GG). This work was partially funded by the DFG SFB645.
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Gutbrod, K., Peisker, H., Dörmann, P. (2021). Direct Infusion Mass Spectrometry for Complex Lipid Analysis. In: Bartels, D., Dörmann, P. (eds) Plant Lipids. Methods in Molecular Biology, vol 2295. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1362-7_7
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DOI: https://doi.org/10.1007/978-1-0716-1362-7_7
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