Abstract
Sterols are an abundant lipid class in the extraplastidic membranes of plant cells. In addition to free sterols, plants contain different conjugated sterols, i.e. sterol esters, sterol glucosides, and acylated sterol glucosides. Sterol lipids can be measured by gas chromatography after separation via thin-layer chromatography. Here, we describe a comprehensive technique based on the quantification of all four sterol classes by direct infusion quadrupole time-of-flight (Q-TOF) mass spectrometry.
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Acknowledgements
This work was supported by an Instrument grant (Forschungsgrossgeräte-Antrag) and by the Forschungsschwerpunkt 1212 of Deutsche Forschungsgemeinschaft (grant Do520/9). We would like to thank Isabel Dombrink, Helga Peisker, and Katharina vom Dorp for their support during method development.
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Wewer, V., Dörmann, P. (2014). Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry. In: Rodríguez-Concepción, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_8
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DOI: https://doi.org/10.1007/978-1-4939-0606-2_8
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