Abstract
Antibodies to autoantigens are implicated in a large number of diseases. Such autoantibodies may cause pathological activation of complement, an ancient humoral recognition and effector system of innate immunity; in addition, complement components or regulators may be target of autoantibodies and cause abnormal complement activation or function. Autoantibodies to complement proteins are in particular involved in kidney diseases. Those binding to complement convertase enzymes can cause enhanced stability of convertases and their increased resistance to regulation, thus promoting complement turnover. Here, we describe an ELISA method to detect factor B autoantibodies that bind to and stabilize the alternative complement pathway C3 convertase enzyme, C3bBb.
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Acknowledgments
The work of the authors is supported by project no. FIEK_16-1-2016-0005, implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the FIEK_16 funding scheme.
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Józsi, M., Uzonyi, B. (2021). Detection of Complement Factor B Autoantibodies by ELISA. In: Roumenina, L.T. (eds) The Complement System. Methods in Molecular Biology, vol 2227. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1016-9_14
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DOI: https://doi.org/10.1007/978-1-0716-1016-9_14
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