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Characterization and Quantification of the Fatty Acid Amidome

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Metabolomics

Part of the book series: Neuromethods ((NM,volume 159))

Abstract

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, we detail the methods we have used to identify, characterize, and quantify a set of fatty acid amides, the fatty acid amidome, from cultured mammalian cells and insects.

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Acknowledgments

This work is dedicated to the memory of the late Dr. Mitchell E. Johnson and has been supported, in part, by grants from the University of South Florida (a Creative Scholarship Grant from the College of Arts and Sciences), the Shirley W. and William L. Griffin Charitable Foundation, the National Institute of Drug Abuse at the National Institutes of Health (R03-DA034323), and the National Institute of General Medical Science of the National Institutes of Health (R15-GM107864) to D.J.M.

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Author notes

  1. Kristen A. Jeffries

    Present address: Agricultural Marketing Service, USDA, Gastonia, NC, USA

  2. Emma K. Farrell

    Present address: The McCrone Group, Westmont, IL, USA

  3. Ryan L. Anderson

    Present address: Department of Biochemistry and Molecular Genetics, University of Colorado - Denver Anschutz Medical Campus, Aurora, CO, USA

  4. Kristen A. Jeffries, Emma K. Farrell, and Ryan L. Anderson contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry, University of South Florida, Tampa, FL, USA

    Kristen A. Jeffries, Emma K. Farrell, Ryan L. Anderson, Gabriela Suarez, Amanda J. Goyette Osborne, Mc Kenzi K. Heide & David J. Merkler

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  1. Kristen A. Jeffries
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  2. Emma K. Farrell
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  3. Ryan L. Anderson
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  4. Gabriela Suarez
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  5. Amanda J. Goyette Osborne
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  6. Mc Kenzi K. Heide
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  7. David J. Merkler
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Corresponding author

Correspondence to David J. Merkler .

Editor information

Editors and Affiliations

  1. Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN, USA

    Paul L. Wood

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Jeffries, K.A. et al. (2021). Characterization and Quantification of the Fatty Acid Amidome. In: Wood, P.L. (eds) Metabolomics . Neuromethods, vol 159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0864-7_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0864-7_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0863-0

  • Online ISBN: 978-1-0716-0864-7

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