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Lipid Extraction and Analysis

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C. elegans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2468))

Abstract

Lipids are major components of cellular membranes and energy stores. Lipids contribute vital structural, energetic, and signaling functions. We have optimized methods to extract and analyze lipids from the nematode Caenorhabditis elegans based on standard methods. Here we describe a method to extract total lipids from C. elegans larvae, adults, or embryos. We describe a thin-layer chromatography method to separate major lipid classes and a gas chromatography method to analyze fatty acid composition from lipid extracts, lipid fractions, or directly from nematode larvae, adults, or embryos.

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Author information

Authors and Affiliations

  1. School of Molecular Biosciences, Washington State University, Pullman, WA, USA

    Henry H. Harrison & Jennifer L. Watts

Authors
  1. Henry H. Harrison
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  2. Jennifer L. Watts
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Corresponding author

Correspondence to Jennifer L. Watts .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, New Jersey Institute of Technology, Newark, NJ, USA

    Gal Haspel

  2. Department of Neuroscience, Brown University, Providence, RI, USA

    Anne C. Hart

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Harrison, H.H., Watts, J.L. (2022). Lipid Extraction and Analysis. In: Haspel, G., Hart, A.C. (eds) C. elegans. Methods in Molecular Biology, vol 2468. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2181-3_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2181-3_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2180-6

  • Online ISBN: 978-1-0716-2181-3

  • eBook Packages: Springer Protocols

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