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Determining the Protective Activity of IDPs Under Partial Dehydration and Freeze-Thaw Conditions

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Intrinsically Disordered Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2141))

Abstract

Unlike for structured proteins, the study of intrinsically disordered proteins (IDPs) requires selection of ad hoc assays and strategies to characterize their dynamic structure and function. Late embryogenesis abundant (LEA) proteins are important plant IDPs closely related to water-deficit stress response. Diverse hypothetical functions have been proposed for LEA proteins, such as membrane stabilizers during cold stress, oxidative regulators acting as ion metal binding molecules, and protein protectants during dehydration and cold/freezing conditions. Here we present two detailed protocols to characterize IDPs with potential protein/enzyme protection activity under partial dehydration and freeze-thaw treatments.

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Author information

Author notes

  1. Cesar L. Cuevas-Velazquez

    Present address: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City, México

  2. David F. Rendón-Luna and Paulette S. Romero-Pérez contributed equally to this work.

Authors and Affiliations

  1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

    David F. Rendón-Luna, Paulette S. Romero-Pérez, Cesar L. Cuevas-Velazquez, José L. Reyes & Alejandra A. Covarrubias

Authors
  1. David F. Rendón-Luna
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  2. Paulette S. Romero-Pérez
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  3. Cesar L. Cuevas-Velazquez
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  4. José L. Reyes
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  5. Alejandra A. Covarrubias
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Corresponding author

Correspondence to Alejandra A. Covarrubias .

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Editors and Affiliations

  1. Structural Biology and NMR Laboratory, Department of Biology, University of Copenhagen, København N, Denmark

    Birthe B. Kragelund

  2. Linderstrøm-Lang Centre for Protein Science, Department of Biology, University of Copenhagen, Copenhagen, Denmark

    Karen Skriver

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Rendón-Luna, D.F., Romero-Pérez, P.S., Cuevas-Velazquez, C.L., Reyes, J.L., Covarrubias, A.A. (2020). Determining the Protective Activity of IDPs Under Partial Dehydration and Freeze-Thaw Conditions. In: Kragelund, B.B., Skriver, K. (eds) Intrinsically Disordered Proteins. Methods in Molecular Biology, vol 2141. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0524-0_26

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  • DOI: https://doi.org/10.1007/978-1-0716-0524-0_26

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0523-3

  • Online ISBN: 978-1-0716-0524-0

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