Abstract
Microbial whole cells are efficient, ecological, and low-cost catalysts that have been successfully applied in the pharmaceutical, environmental, and alimentary industries, among others.
Microorganism immobilization is a good way to carry out the bioprocess under preparative conditions. The main advantages of this methodology lie in their high operational stability, easy upstream separation, and bioprocess scale-up feasibility.
Cell entrapment is the most widely used technique for whole cell immobilization. This technique—in which the cells are included within a rigid network—is porous enough to allow the diffusion of substrates and products, protects the selected microorganism from the reaction medium, and has high immobilization efficiency (100% in most cases).
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Acknowledgments
This work was supported by Agencia Nacional de Promoción Científica y Tecnológica, Universidad Nacional de Quilmes and CONICET.
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Trelles, J.A., Rivero, C.W. (2020). Whole Cell Entrapment Techniques. In: Guisan, J., Bolivar, J., López-Gallego, F., Rocha-Martín, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 2100. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0215-7_25
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DOI: https://doi.org/10.1007/978-1-0716-0215-7_25
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