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Quantitative Jasmonate Profiling Using a High-Throughput UPLC-NanoESI-MS/MS Method

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Jasmonate in Plant Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2085))

Abstract

Jasmonic acid (JA) and its many derivatives-collectively referred as jasmonates-occur ubiquitously in land plants and regulate a wide range of stress-responses and development. Measuring these signaling compounds is complicated by the large number of jasmonate derivatives and the comparatively low concentration of these metabolites in plant tissues. We, here, present a selective and sensitive method consisting of a two-phase extraction coupled with liquid chromatography, nanoelectrospray ionization, and mass spectrometry to determine jasmonate levels in tissues and fluids of various plant species. The application of stable deuterium-labelled standards in combination with authentic standards allows the absolute quantification of a multitude of jasmonates and, additionally, the semi-quantitative analysis of further metabolites from the jasmonate pathway.

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Acknowledgments

We are very grateful to Tim Iven for improving the phytohormone analysis platform and Krzysztof Zienkiewicz for testing the robustness of the method. We thank Sabine Freitag and Pia Meyer for excellent assistance. This research has been funded by the DFG (ZUK 45/2010; INST 186/822-1).

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Authors and Affiliations

  1. Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, University of Goettingen, Goettingen, Germany

    Cornelia Herrfurth & Ivo Feussner

  2. Service Unit for Metabolomics and Lipidomics, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, Germany

    Cornelia Herrfurth & Ivo Feussner

  3. Department of Plant Biochemistry, Goettingen Center for Molecular Biosciences (GZMB), University of Goettingen, Goettingen, Germany

    Ivo Feussner

Authors
  1. Cornelia Herrfurth
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  2. Ivo Feussner
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Correspondence to Ivo Feussner .

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Editors and Affiliations

  1. UMR DIADE - IRD, Montpellier, France

    Antony Champion

  2. UMR DIADE - IRD, Montpellier, France

    Laurent Laplaze

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Herrfurth, C., Feussner, I. (2020). Quantitative Jasmonate Profiling Using a High-Throughput UPLC-NanoESI-MS/MS Method. In: Champion, A., Laplaze, L. (eds) Jasmonate in Plant Biology. Methods in Molecular Biology, vol 2085. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0142-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0142-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0141-9

  • Online ISBN: 978-1-0716-0142-6

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