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Mutants That Affect Recoding

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Recoding: Expansion of Decoding Rules Enriches Gene Expression

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 24))

Abstract

Fast, accurate translation is a hallmark of protein synthesis. However, recoding systems subvert the normal decoding mechanisms to redefine codons or shift translation into alternate reading frames. Genetic analyses in yeast and bacteria have enhanced our understanding of non-standard decoding and of normal decoding processes. Recent crystallographic efforts have provided unprecedented insights into the mechanisms underlying translation and the selection of cognate tRNAs. Ribosomal components and factors involved in decoding have also been identified genetically, through selections for mutants that alter the fidelity of protein synthesis, or impact upon various recoding phenomena. The availability of high-resolution structures of ribosomal complexes together with advances in biochemistry now allow the effects of many of these mutant components to be explained in molecular terms.

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Acknowledgments

Work in the authors’ laboratories was supported by grants GM058859 and AI064307 from the National Institutes of Health (to J.D.D.) and MCB0745025 from the National Science Foundation (to M.OC.).

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Authors and Affiliations

  1. Department of Cell Biology and Molecular Genetics, 2135 Microbiology Building, University of Maryland, 20742, College Park, MD, USA

    Jonathan D. Dinman

  2. School of Biological Sciences, University of Missouri-Kansas City, 64110, Kansas City, MO, USA

    Michael O’Connor

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  1. Jonathan D. Dinman
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  2. Michael O’Connor
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Correspondence to Jonathan D. Dinman .

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Editors and Affiliations

  1. Molecular Biology Program, University of Utah, N. 2030 E. 15, Salt Late City, 84112-5330, U.S.A.

    John F. Atkins

  2. Dept. Bioengineering, University of Utah, Salt Lake City, 84112, U.S.A.

    Raymond F. Gesteland

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Dinman, J.D., O’Connor, M. (2010). Mutants That Affect Recoding. In: Atkins, J., Gesteland, R. (eds) Recoding: Expansion of Decoding Rules Enriches Gene Expression. Nucleic Acids and Molecular Biology, vol 24. Springer, New York, NY. https://doi.org/10.1007/978-0-387-89382-2_15

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