Abstract
HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent controlled differentiation using calcium induction.
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Acknowledgments
Much of our work with HaCaT cells was supported by NIH grant CA089289. We thank all the previous members of the Wilson lab for their help with development of the HaCaT protocols, particularly Drs. Adeline Deyrieux and Phillip Heaton.
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© 2013 Springer Science+Business Media New York
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Wilson, V.G. (2013). Growth and Differentiation of HaCaT Keratinocytes. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 1195. Springer, New York, NY. https://doi.org/10.1007/7651_2013_42
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DOI: https://doi.org/10.1007/7651_2013_42
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