Abstract
The possibility of turning one somatic cell type into another may in the long run have beneficial applications in regenerative medicine. Somatic cell nuclear transfer (therapeutic cloning) may offer this possibility; however, ethical guidelines prevent application of this technology in many in countries. As a result, alternative approaches are being developed for altering cell fate. This communication discusses recent non-nuclear transfer-based in vitro approaches for reprogramming cells and enhancing their potential for differentiation toward various lineages.
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References
Akashi K, He X, Chen J, Iwasaki H, Niu C, Steenhard B, Zhang J, Haug J, Li L (2003) Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis. Blood 101:383–389
Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell-Badge R (2003) Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 17:126–140
Blelloch RH, Hochedlinger K, Yamada Y, Brennan C, Kim M, Mintz B, Chin L, Jaenisch R (2004) Nuclear cloning of embryonal carcinoma cells. Proc Natl Acad Sci U S A 101:13985–13990
Boquest AC, Shahdadfar A, Fronsdal K, Sigurjonsson O, Tunheim SH, Collas P, Brinchmann JE (2005) Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression following in vitro cell culture. Mol Biol Cell 16:1131–1141
Byrne JA, Simonsson S, Western PS, Gurdon JB (2003) Nuclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes. Curr Biol 13:1206–1213
Chambers I, Smith A (2004) Self-renewal of teratocarcinoma and embryonic stem cells. Oncogene 23:7150–7160
Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce DLF, Robl JM (1998) Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotechnol 16:642–646
Collas P, Håkelien AM (2003) Teaching cells new tricks. Trends Biotechnol 21:354–361
Flasza M, Shering AF, Smith K, Andrews PW, Talley P, Johnson PA (2003) Reprogramming in inter-species embryonal carcinoma-somatic cell hybrids induces expression of pluripotency and differentiation markers. Cloning Stem Cells 5:339–354
Gaustad KG, Boquest AC, Anderson BE, Gerdes AM, Collas P (2004) Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes. Biochem Biophys Res Commun 314:420–427
Gurdon JB, Byrne JA (2003) The first half-century of nuclear transplantation. Proc Natl Acad Sci U S A 100:8048–8052
Håkelien AM, Landsverk HB, Robl JM, Skålhegg BS, Collas P (2002) Reprogramming fibroblasts to express T-cell functions using cell extracts. Nat Biotechnol 20:460–466
Håkelien AM, Gaustad KG, Collas P (2004) Transient alteration of cell fate using a nuclear and cytoplasmic extract of an insulinoma cell line. Biochem Biophys Res Commun 316:834–841
Håkelien AM, Gaustad KG, Taranger CK, Skalhegg BS, Kuntziger T, Collas P (2005) Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression. Exp Cell Res 309:32–47
Hansis C, Barreto G, Maltry N, Niehrs C (2004) Nuclear reprogramming of human somatic cells by xenopus egg extract requires BRG1. Curr Biol 14:1475–1480
Hutchison CJ, Worman HJ (2004) A-type lamins: guardians of the soma? Nat Cell Biol 6:1062–1067
Kikyo N, Wade PA, Guschin D, Ge H, Wolffe AP (2000) Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI. Science 289:2360–2362
Klevecz RR, Bolen J, Forrest G, Murray DB (2004) A genomewide oscillation in transcription gates DNA replication and cell cycle. Proc Natl Acad Sci U S A 101:1200–1205
Landsverk HB, Håkelien AM, Küntziger T, Robl JM, Skålhegg BS, Collas P (2002) Reprogrammed gene expression in a somatic cell-free extract. EMBO Rep 3:384–389
Mathieu O, Bender J (2004) RNA-directed DNA methylation. J Cell Sci 117:4881–4888
Matzke MA, Birchler JA (2005) RNAi-mediated pathways in the nucleus. Nat Rev Genet 6:24–35
McGann CJ, Odelberg SJ, Keating MT (2001) Mammalian myotube dedifferentiation induced by newt regeneration extract. Proc Natl Acad Sci U S A 98:13699–13704
Miklos GL, Maleszka R (2004) Microarray reality checks in the context of a complex disease. Nat Biotechnol 22:615–621
Morgan HD, Dean W, Coker HA, Reik W, Petersen-Mahrt SK (2004) Activation-induced cytidine deaminase deaminates 5-methylcytosine in DNA and is expressed in pluripotent tissues: implications for epigenetic reprogramming. J Biol Chem 279:52353–52360
Munsie MJ, Michalska AE, O’Brien CM, Trounson AO, Pera MF, Mountford PS (2000) Isolation of pluripotent embryonic stem cells from reprogrammed adult mouse somatic cell nuclei. Curr Biol 10:989–992
Nishimoto M, Fukushima A, Okuda A, Muramatsu M (1999) The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2. Mol Cell Biol 19:5453–5465
Okuda A, Fukushima A, Nishimoto M, Orimo A, Yamagishi T, Nabeshima Y, Kuro-o M, Nabeshima Y, Boon K, Keaveney M, Stunnenberg HG, Muramatsu M (1998) UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. EMBO J 17:2019–2032
Paulsson J (2004) Summing up the noise in gene networks. Nature 427:415–418
Pells S, Di Domenico AI, Callagher EJ, McWhir J (2002) Multipotentiality of neuronal cells after spontaneous fusion with embryonic stem cells and nuclear reprogramming in vitro. Cloning Stem Cells 4:331–338
Qin M, Tai G, Collas P, Polak JM, Bishop AE (2005) Cell extract-derived differentiation of embryonic stem cells. Stem Cells 23:712–718
Ramchandani S, Bhattacharya SK, Cervoni N, Szyf M (1999) DNA methylation is a reversible biological signal. Proc Natl Acad Sci U S A 96:6107–6112
Simonsson S, Gurdon J (2004) DNA demethylation is necessary for the epigenetic reprogramming of somatic cell nuclei. Nat Cell Biol 6:984–990
Sullivan EJ, Kasinathan S, Kasinathan P, Robl JM, Collas P (2004) Cloned calves from chromatin remodeled in vitro. Biol Reprod 70:146–153
Tada M, Tada T, Lefebvre L, Barton SC, Surani MA (1997) Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells. EMBO J 16:6510–6520
Tada M, Takahama Y, Abe K, Nakastuji N, Tada T (2001) Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol 11:1553–1558
Terada N, Hamazaki T, Oka M, Hoki M, Mastalerz DM, Nakano Y, Meyer EM, Morel L, Petersen BE, Scott EW (2002) Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature 416:542–545
Tomioka M, Nishimoto M, Miyagi S, Katayanagi T, Fukui N, Niwa H, Muramatsu M, Okuda A (2002) Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nucleic Acids Res 30:3202–3213
Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P (2001) Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Science 292:740–743
Wilmut I, Beaujean N, De Sousa PA, Dinnyes A, King TJ, Paterson LA, Wells DN, Young LE (2002) Somatic cell nuclear transfer. Nature 419:583–586
Woodbury D, Reynolds K, Black IB (2002) Adult bone marrow stromal stem cells express germline, ectodermal, endodermal, and mesodermal genes prior to neurogenesis. J Neurosci Res 69:908–917
Ying QL, Nichols J, Evans EP, Smith AG (2002) Changing potency by spontaneous fusion. Nature 416:545–548
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Collas, P., Taranger, C.K. (2006). Toward Reprogramming Cells to Pluripotency. In: Morser, J., Nishikawa, S.I., Schöler, H.R. (eds) Stem Cells in Reproduction and in the Brain. Ernst Schering Research Foundation Workshop, vol 60. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-31437-7_5
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DOI: https://doi.org/10.1007/3-540-31437-7_5
Publisher Name: Springer, Berlin, Heidelberg
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