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Adaptions of the mineral composition of tissue culture media on the basis of plant elemental analysis and composition of hydroponic substrates

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Liquid Culture Systems for in vitro Plant Propagation

Abstract

For the improvement of in vitro propagation protocols of Gerbera and Cymbidium, we adapted the macronutrients according to the elemental composition of adult leaves. In comparison with normally used media, in particular, the Ca and P concentrations were much higher. Using the adapted media, for both crops growth was much improved. The relative concentrations of macronutrients in these modified media formulations is much more in accordance with the mineral composition of nutrient solutions used in hydroponic cultures. For Gerbera, we also made adaptations to the micronutrients according to the elemental composition of nutrient media in hydroponics (viz., increased Cu-content and decreased Mn-content). This resulted in additional improvement of growth; by this adaptation, the plants became larger and also greener (only with the Cu adaptation).

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Correspondence to Han Bouman .

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Bouman, H., Tiekstra, A. (2005). Adaptions of the mineral composition of tissue culture media on the basis of plant elemental analysis and composition of hydroponic substrates. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_37

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