Abstract
Experiments to characterise long-term embryogenic suspension cultures of Gentiana cruciata (L.) and G. tibetica (King) are reported. Cell suspensions of both species differed in the percentage of five selected fractions of cell aggregates, as well as in fresh and dry mass during three years of culture. In G. tibetica the ratio of cells in phase G2 to G1 was higher than in G. cruciata. The response of suspension cultures to GA3 (at 0, 1.49 or 2.89 µmol), kinetin (at 0, 2.32, 4.64 or 9.28 µmol) and adenine sulphate (at 0 or 434 µmol) was studied. The increase of kinetin concentration stimulated embryo production in suspensions of G. tibetica. Somatic embryo production in G. tibetica was significantly higher than in G. cruciata. In G. tibetica, the aggregate fraction >450 µm was at least four times more productive than the same fraction in G. cruciata suspensions.
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Mikuła, A., Rybczyński, J.J., Skierski, J., Latkowska, M.J., Fiuk, A. (2005). Somatic embryogenesis of Gentiana genus IV.: Characterisation of Gentiana cruciata and Gentiana tibetica embryogenic cell suspensions. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_26
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DOI: https://doi.org/10.1007/1-4020-3200-5_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
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