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Dark Field Microscopic Sensitive Detection of Amyloid Fibrils Using Gold Nanoparticles Modified with Antibody

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Abstract

Dark field microscopy (DFM) was employed to detect amyloid β (Aβ) fibrils-induced gold nanoparticle (AuNP) aggregation at the single-particle level, with a detection limit of 40 pM fibrils. The sensitivity of this method is higher than that of the current fibril-specific detection method using probe dye, such as thioflavin T, for which sub-μM level of fibrils are necessary. This study further proved the potential application of DFM in the analytical methods based on AuNP aggregation.

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Acknowledgments

We thank Dr. Kazuo Hosokawa (Bioengineering Lab., RIKEN) for help with LOD calculation. T. B. is a RIKEN International Program Associate. This work was financially supported by RIKEN (Molecular Systems Research) and JSPS KAKENHI (Grant Nos. 24570143 and 25286015).

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Author notes

  1. Tamotsu Zako

    Present address: T. Z. present address: Department of Chemistry and Biology, Graduate School of Science and Engineering, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime, 790-8577, Japan

Authors and Affiliations

  1. Department of Advanced Materials, Science, School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha Kashiwa, Chiba, 277-8561, Japan

    Tong Bu & Mizuo Maeda

  2. Bioengineering Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Tong Bu, Tamotsu Zako & Mizuo Maeda

Authors
  1. Tong Bu
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  2. Tamotsu Zako
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  3. Mizuo Maeda
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Correspondence to Tamotsu Zako.

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Bu, T., Zako, T. & Maeda, M. Dark Field Microscopic Sensitive Detection of Amyloid Fibrils Using Gold Nanoparticles Modified with Antibody. ANAL. SCI. 32, 307–311 (2016). https://doi.org/10.2116/analsci.32.307

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  • DOI: https://doi.org/10.2116/analsci.32.307

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