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Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membranes

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Abstract

A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (•OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of •OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with •OH and increased the fluorescence intensity, depending on the concentration of •OH. The increase in the fluorescence intensity induced by •OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to •OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect •OH efficiently.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi, Fukuoka, 819-0395, Japan

    Nobuaki Soh, Koji Makihara, Tomoyuki Ariyoshi, Daisuke Seto, Tomoharu Maki, Hizuru Nakajima, Koji Nakano & Toshihiko Imato

Authors
  1. Nobuaki Soh
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  2. Koji Makihara
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  3. Tomoyuki Ariyoshi
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  4. Daisuke Seto
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  5. Tomoharu Maki
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  6. Hizuru Nakajima
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  7. Koji Nakano
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  8. Toshihiko Imato
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Correspondence to Nobuaki Soh.

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Soh, N., Makihara, K., Ariyoshi, T. et al. Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membranes. ANAL. SCI. 24, 293–296 (2008). https://doi.org/10.2116/analsci.24.293

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  • DOI: https://doi.org/10.2116/analsci.24.293

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