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Determination of 3-Methyl-quinoxaline-2-carboxylic Acid and Quinoxaline-2-carboxylic Acid in Pork Based on a Background Fluorescence Quenching Immunochromatographic Assay

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Abstract

A novel rapid method based on a background fluorescence quenching immunochromatographic assay (bFQICA) was established to achieve simultaneously the quantitative detection of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) and quinoxaline-2-carboxylic acid (QCA), which were efficiently extracted and enriched 4 times using immunomagnetic beads from pork. The analysis of field pork samples by bFQICA was in accordance with that of LC-MS/MS; especially, the proposed bFQICA exhibited great advantages in convenience and efficiency, which only takes 30 min for the detection of MQCA and QCA.

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Acknowledgments

This research was funded by the National Natural Science Foundation of China, grant number 31672605; Natural Science Foundation of Chongqing, China, grant number cstc2018jcyjAX0242 and cstc2017jcyjAX0313; China Postdoctoral Science Foundation, grant number 2016M590855; Chongqing Postdoctoral Science Foundation Special Funded Project, grant number Xm2017074.

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Correspondence to Xiaoqi Tao.

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Wan, X., Wang, X. & Tao, X. Determination of 3-Methyl-quinoxaline-2-carboxylic Acid and Quinoxaline-2-carboxylic Acid in Pork Based on a Background Fluorescence Quenching Immunochromatographic Assay. ANAL. SCI. 36, 783–785 (2020). https://doi.org/10.2116/analsci.20C008

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  • DOI: https://doi.org/10.2116/analsci.20C008

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