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Provision of Ultrasensitive Quantitative Gold Immunochromatography for Rapid Monitoring of Olaquindox in Animal Feed and Water Samples

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Abstract

Olaquindox (OLA), used as a medicinal feed additive, has been put under ban due to hazard concerns over animal-derived food security. In this study, a simple, rapid, ultrasensitive, and quantitative gold immunochromatography assay (GICA) was established to analyze OLA in animal feed samples and surface water samples to monitor food security. Various trying has been experimented to improve the sensitivity. The IC50 of the optimized method is 3.35 μg L−1 for feedstuff and 0.35 μg L−1 for environmental water. The recoveries ranged from 77.33 to 86.91 % (CV <23.62 %) for spiked feedstuff and 96.30 to 117.83 % (CV <22.51 %) for spiked water samples. Then, the developed GICA was applied to animal feed and field water, followed by confirmation with ELISA and the consistency of results indicated that the developed GICA could be applied for rapid screening of OLA in real samples. Compared with previous assay, the developed GICA in this study was more sensitive and more rapid, which exhibited broader prospect to supervise the animal products and water rapidly.

Schematic illustration of the test strip

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Acknowledgments

This study was supported by the Special Research Project of Public Welfare Quality Testing Industry (2012104003) and the National Science & Technology Pillar Program during the 12th 5-year Plan Period (2015BAK36B03). And the authors appreciate the cooperation of other faculty members in the Department of Pharmacology and Toxicology of the College of Veterinary Medicine at China Agricultural University.

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Authors and Affiliations

  1. National Reference Laboratory for Veterinary Drug Residues, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China

    Xingyao Pei, Qi Wang, Jie Xie, Sanlei Xie, Tao Peng, Cheng Wang, Yuanze Sun & Haiyang Jiang

  2. Beijing WDWK Biotechnological Co., Ltd, Beijing, 100095, China

    Xiangmei Li

Authors
  1. Xingyao Pei
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  2. Qi Wang
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  3. Xiangmei Li
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  4. Jie Xie
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  5. Sanlei Xie
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  6. Tao Peng
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  7. Cheng Wang
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  8. Yuanze Sun
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  9. Haiyang Jiang
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Corresponding author

Correspondence to Haiyang Jiang.

Ethics declarations

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of Interest

Xingyao Pei declares that she has no conflict of interest. Qi Wang declares that she has no conflict of interest. Xiangmei Li declares that she has no conflict of interest. Jie Xie declares that she has no conflict of interest. Sanlei Xie declares that he has no conflict of interest. Tao Peng declares that he has no conflict of interest. Cheng Wang declares that he has no conflict of interest. Yuanze Sun declares that she has no conflict of interest. Haiyang Jiang declares that he has no conflict of interest.

Informed Consent

Not applicable (It is not applicable on the study).

Funding

This study was funded by the Special Research Project of Public Welfare Quality Testing Industry (2012104003) and the National Science & Technology Pillar Program during the 12th 5-year Plan Period (2015BAK36B03).

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Pei, X., Wang, Q., Li, X. et al. Provision of Ultrasensitive Quantitative Gold Immunochromatography for Rapid Monitoring of Olaquindox in Animal Feed and Water Samples. Food Anal. Methods 9, 1919–1927 (2016). https://doi.org/10.1007/s12161-015-0360-y

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  • DOI: https://doi.org/10.1007/s12161-015-0360-y

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