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Application of Polyaniline/Sol-Gel Derived Tetraethylorthosilicate Films to an Amperometric Lactate Biosensor

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Abstract

The electrochemical entrapment of polyaniline (PANI) onto sol-gel derived tetraethylorthosilicate (TEOS) films deposited onto indium-tin-oxide (ITO) coated glass has been utilized for immobilization of lactate dehydrogenase (LDH). The performance of these sol-gel/PANI/LDH electrodes has been investigated as a function of the lactate concentration, applied potential, pH of the medium and interferents. The amperometric response of the electrodes under optimum conditions exhibited a linear relationship from 1 mM to 4 mM. An attempt has been made to extend the linearity up to 10 mM for lactate by coating an external layer of polyvinyl chloride (PVC) over the sol-gel/PANI/LDH electrodes with a correlation coefficient of 0.89. These sol-gel/PANI/LDH electrodes have a response time of about 60 s, a shelf life of about 8 weeks at 0–4°C and have implications in a lactate biosensor.

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Authors and Affiliations

  1. Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi, 110012, India

    Asha Chaubey, K. K. Pande & B. D. Malhotra

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  1. Asha Chaubey
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  2. K. K. Pande
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  3. B. D. Malhotra
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Chaubey, A., Pande, K.K. & Malhotra, B.D. Application of Polyaniline/Sol-Gel Derived Tetraethylorthosilicate Films to an Amperometric Lactate Biosensor. ANAL. SCI. 19, 1477–1480 (2003). https://doi.org/10.2116/analsci.19.1477

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  • DOI: https://doi.org/10.2116/analsci.19.1477

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