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Antiinfluenza virus activity of a bacteriocin produced by Lactobacillus delbrueckii

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Abstract

A novel antibacterial substance produced by Lactobacillus delbrueckii has been isolated and characterized (1). The inhibitory agent corresponded to the criteria for bacteriocins. It was active against lactic acid bacteria (LAB) species and several food-borne pathogens. The cell-free supernatant was purified by HPLC gel-filtration. Three preparations at different purification steps were tested for activity on the reproduction of influenza virus A/chicken/Germany, strain Weybridge (H7N7) and strain Rostock (H7N1) in cell cultures of chicken embryo fibroblasts (CEF). The inhibitory effect was shown to be highly selective and specific. Expression of viral glycoproteins hemagglutinin, neuraminidase, and nucleoprotein on the surface of infected cells, virus-induced cytopathic effect, infectious virus yield, and hemagglutinin production were all reduced at nontoxic concentrations of the crude preparation (B1). B1 did not protect cells from infection, did not affect adsorption, and slightly inhibited viral penetration into infected cells. The purification did not enhance the cellular toxicity and increased about 870-fold the virus-inhibitory activity. No inactivating effect on extracellular virus was found.

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Authors and Affiliations

  1. Institute of Microbiology, Bulgarian Academy of Sciences, Acad. Georgy Bonchev str. bl. 26, 1113, Sofia, Bulgaria

    Julia Serkedjieva

  2. Department of Microbiology, Faculty of Biology, Sofia University, Bulgaria

    Svetla Danova & Iskra Ivanova

Authors
  1. Julia Serkedjieva
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  2. Svetla Danova
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  3. Iskra Ivanova
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Correspondence to Julia Serkedjieva.

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Serkedjieva, J., Danova, S. & Ivanova, I. Antiinfluenza virus activity of a bacteriocin produced by Lactobacillus delbrueckii . Appl Biochem Biotechnol 88, 285–298 (2000). https://doi.org/10.1385/ABAB:88:1-3:285

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  • DOI: https://doi.org/10.1385/ABAB:88:1-3:285

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